世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2014年
8期
1059-1062
,共4页
附麻平喘方%作用%机制%实验研究
附痳平喘方%作用%機製%實驗研究
부마평천방%작용%궤제%실험연구
Fuma Pingchuan Prescription%Effect%Mechanism%Experimental Study
目的:实验探讨附麻平喘方对哮喘模型大鼠的影响并探讨其作用机制。方法:选用SPF级清洁雄性大鼠60只,采用注射卵蛋白(OVA)与氢氧化铝致敏、OVA雾化吸入激发来复制哮喘动物模型,造模后将其随机分为5组:模型组(等容量蒸馏水)、附麻防喘片组(377.5 mg/kg)、附麻平喘方高剂量组(49.68 g/kg)、附麻平喘方中剂量组(24.84 g/kg)、附麻平喘方低剂量组(12.42 g/kg),每组10只。并于致敏2周后开始灌胃给药8周。末次给药后,采用ELISA法检测血浆ACTH,血清CORT、IL-4、IFN-γ及IgE的含量;取肺脏,一侧做HE染色,另一侧采用免疫组化法检测肺组织内β! AR的含量。结果:附麻平喘方能有效缓解模型大鼠哮喘的症状,减轻肺组织病变程度,同时显著升高血清CORT,血浆ACTH,肺组织β! AR的水平。结论:附麻平喘方可改善哮喘模型大鼠的症状,其机制可能与改善HPA轴及免疫功能有关。
目的:實驗探討附痳平喘方對哮喘模型大鼠的影響併探討其作用機製。方法:選用SPF級清潔雄性大鼠60隻,採用註射卵蛋白(OVA)與氫氧化鋁緻敏、OVA霧化吸入激髮來複製哮喘動物模型,造模後將其隨機分為5組:模型組(等容量蒸餾水)、附痳防喘片組(377.5 mg/kg)、附痳平喘方高劑量組(49.68 g/kg)、附痳平喘方中劑量組(24.84 g/kg)、附痳平喘方低劑量組(12.42 g/kg),每組10隻。併于緻敏2週後開始灌胃給藥8週。末次給藥後,採用ELISA法檢測血漿ACTH,血清CORT、IL-4、IFN-γ及IgE的含量;取肺髒,一側做HE染色,另一側採用免疫組化法檢測肺組織內β! AR的含量。結果:附痳平喘方能有效緩解模型大鼠哮喘的癥狀,減輕肺組織病變程度,同時顯著升高血清CORT,血漿ACTH,肺組織β! AR的水平。結論:附痳平喘方可改善哮喘模型大鼠的癥狀,其機製可能與改善HPA軸及免疫功能有關。
목적:실험탐토부마평천방대효천모형대서적영향병탐토기작용궤제。방법:선용SPF급청길웅성대서60지,채용주사란단백(OVA)여경양화려치민、OVA무화흡입격발래복제효천동물모형,조모후장기수궤분위5조:모형조(등용량증류수)、부마방천편조(377.5 mg/kg)、부마평천방고제량조(49.68 g/kg)、부마평천방중제량조(24.84 g/kg)、부마평천방저제량조(12.42 g/kg),매조10지。병우치민2주후개시관위급약8주。말차급약후,채용ELISA법검측혈장ACTH,혈청CORT、IL-4、IFN-γ급IgE적함량;취폐장,일측주HE염색,령일측채용면역조화법검측폐조직내β! AR적함량。결과:부마평천방능유효완해모형대서효천적증상,감경폐조직병변정도,동시현저승고혈청CORT,혈장ACTH,폐조직β! AR적수평。결론:부마평천방가개선효천모형대서적증상,기궤제가능여개선HPA축급면역공능유관。
Objective:To examine the effect of the Fuma Pingchuan Prescription for Asthma in Experimental Rats and to study its mech-anism.Methods:Sixty Clean male rats of SPF.Except the blank control group,all the rats were injected with ovalbumin(OVA)and alu-minum hydroxide sensitization,OVA inhalation excited to replicate animal models of asthma.The experimental rats were divided into five groups:the model group(such as volume of distilled water),positive compare group(377.5 mg/kg),Fuma Pingchuan Prescription high dose group(49.68 g/kg),middle dose group(24.84 g/kg)and low dose group(12.42 g/kg).Each group consists of ten rats.The rats started to receive intragastric administration 2 weeks after sensitization,1 dose each day in 8 weeks.After the last administration detected by ELISA in plasma ACTH,serum CORT,IL-4、IFN-γand IgE levels.Staining the tissue in one side of the lung with HE and detec-ting β-AR content.In the other side by immunohistochemistry.Results:Fuma Pingchuan Prescription can effectively alleviate asthma, reduce asthma symptoms and improve lung tissue morphology,while significantly elevated serum CORT,plasma ACTH,lung tissue β-AR levels.Conclusion:Fuma Pingchuan Prescription can improve asthma symptoms of model rats,which may be related to the improved functions of HPA axis and immune.