CAJ | 학술논문

目的：观察转化生长因子β1（TGF-β1）及活化素受体样激酶1（ALK1）在人脑胶质瘤细胞中的表达及其在胶质细胞瘤病理分级中的意义。方法采用半定量RT-PCR、Western blot及免疫组织化学染色等方法检测32例人脑胶质瘤及3例正常脑组织中TGF-β1及ALK1 mRNA及蛋白的表达水平。结果人脑胶质瘤细胞中存在 TGF-β1及ALK1 mRNA和蛋白表达。与正常组比较，高级别胶质瘤组TGF-β1及ALK1 mRNA和蛋白表达均显著升高（P＜0．05），其中ALK1的高表达与胶质瘤病理级别呈明显正相关（r＝0．297，P＜0．05）。结论人脑胶质细胞瘤中，ALK1可能参与了胶质瘤的恶变过程。
목적：관찰전화생장인자β1（TGF-β1）급활화소수체양격매1（ALK1）재인뇌효질류세포중적표체급기재효질세포류병리분급중적의의。방법채용반정량RT-PCR、Western blot급면역조직화학염색등방법검측32례인뇌효질류급3례정상뇌조직중TGF-β1급ALK1 mRNA급단백적표체수평。결과인뇌효질류세포중존재 TGF-β1급ALK1 mRNA화단백표체。여정상조비교，고급별효질류조TGF-β1급ALK1 mRNA화단백표체균현저승고（P＜0．05），기중ALK1적고표체여효질류병리급별정명현정상관（r＝0．297，P＜0．05）。결론인뇌효질세포류중，ALK1가능삼여료효질류적악변과정。
Objective To study the expression of transforming growth factor beta1(TGF-β1)and its type I receptors activin-like kinase 1(ALK1)in the human brain glioma and estimate the significance in the pathogenisis and development of human glioma . Methods The mRNA and protein expressions of TGF-β1 and ALK1 were detected with semiquantitative RT-PCR ,Western blot and Immunohistochemistry in 3 normal brain tissues and 32 patiens with human glioma .Results ALK1 and TGF-β1 mRNA and protein sometimes had co-expression in human gliomas tissues .Compared with those normal brain tissues ,the expression of TGF-β1 and ALK1 mRNA and protein in high grade gliomas were significantly increased (P<0 .05) ,the overexpression of ALK1 in human gliomas was demonstrated significant positive correlation with the pathological grades of gliomas (r=0 .297 ,P<0 .05) .Conclusion The overexpression of ALK1 may play important role in the development of human brain glioma .