重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
24期
3124-3126
,共3页
蔡佳%张曼%赵旭宏%雷婷%孟倩%周慧
蔡佳%張曼%趙旭宏%雷婷%孟倩%週慧
채가%장만%조욱굉%뢰정%맹천%주혜
妊娠%尿液分析%促甲状腺激素%游离三碘甲状腺原氨酸%游离甲状腺素
妊娠%尿液分析%促甲狀腺激素%遊離三碘甲狀腺原氨痠%遊離甲狀腺素
임신%뇨액분석%촉갑상선격소%유리삼전갑상선원안산%유리갑상선소
pregnancy%urinalysis%thyrotropin%free triiodothyronine%free tetraiodothyronine
目的：分析妊娠期妇女尿液甲状腺功能相关激素含量的变化，为进一步研究通过检测尿液监测其甲状腺功能状态奠定基础。方法随机选择30名健康产科门诊志愿者，孕9～12周起直至分娩，每4周收集1次各孕妇的随机尿标本。电化学发光免疫分析法测定尿促甲状腺激素（uTSH）、尿游离三碘甲状腺原氨酸（uFT3）和尿游离甲状腺素（uFT4），酶免疫分析法测定尿视黄醇结合蛋白（uRBP）浓度。经uRBP校正后，统计分析孕期uTSH与uRBP比值（uTSH/uRBP）、uFT3与uRBP比值（uFT3/uRBP）和uFT4与uRBP比值（uFT4/uRBP）的含量变化。结果该研究得到各妊娠时段尿液中甲状腺功能相关激素的中值浓度。统计学分析表明，各妊娠时段孕妇 uFT3/uRBP和 uFT4/uRBP含量比较差异均有统计学意义（ F＝6．222，P＜0．05；F＝5．078，P＜0．05），且呈线性趋势变化（F＝27．480，P＜0．05；F＝23．959，P＜0．05）。uTSH/uRBP的含量在各妊娠时段差异无统计学意义（F＝2．731，P＝0．054）。结论经uRBP校正后，健康孕妇各妊娠时段uFT3和uFT4的含量呈线性趋势下降，uTSH的含量无明显变化趋势。
目的：分析妊娠期婦女尿液甲狀腺功能相關激素含量的變化，為進一步研究通過檢測尿液鑑測其甲狀腺功能狀態奠定基礎。方法隨機選擇30名健康產科門診誌願者，孕9～12週起直至分娩，每4週收集1次各孕婦的隨機尿標本。電化學髮光免疫分析法測定尿促甲狀腺激素（uTSH）、尿遊離三碘甲狀腺原氨痠（uFT3）和尿遊離甲狀腺素（uFT4），酶免疫分析法測定尿視黃醇結閤蛋白（uRBP）濃度。經uRBP校正後，統計分析孕期uTSH與uRBP比值（uTSH/uRBP）、uFT3與uRBP比值（uFT3/uRBP）和uFT4與uRBP比值（uFT4/uRBP）的含量變化。結果該研究得到各妊娠時段尿液中甲狀腺功能相關激素的中值濃度。統計學分析錶明，各妊娠時段孕婦 uFT3/uRBP和 uFT4/uRBP含量比較差異均有統計學意義（ F＝6．222，P＜0．05；F＝5．078，P＜0．05），且呈線性趨勢變化（F＝27．480，P＜0．05；F＝23．959，P＜0．05）。uTSH/uRBP的含量在各妊娠時段差異無統計學意義（F＝2．731，P＝0．054）。結論經uRBP校正後，健康孕婦各妊娠時段uFT3和uFT4的含量呈線性趨勢下降，uTSH的含量無明顯變化趨勢。
목적：분석임신기부녀뇨액갑상선공능상관격소함량적변화，위진일보연구통과검측뇨액감측기갑상선공능상태전정기출。방법수궤선택30명건강산과문진지원자，잉9～12주기직지분면，매4주수집1차각잉부적수궤뇨표본。전화학발광면역분석법측정뇨촉갑상선격소（uTSH）、뇨유리삼전갑상선원안산（uFT3）화뇨유리갑상선소（uFT4），매면역분석법측정뇨시황순결합단백（uRBP）농도。경uRBP교정후，통계분석잉기uTSH여uRBP비치（uTSH/uRBP）、uFT3여uRBP비치（uFT3/uRBP）화uFT4여uRBP비치（uFT4/uRBP）적함량변화。결과해연구득도각임신시단뇨액중갑상선공능상관격소적중치농도。통계학분석표명，각임신시단잉부 uFT3/uRBP화 uFT4/uRBP함량비교차이균유통계학의의（ F＝6．222，P＜0．05；F＝5．078，P＜0．05），차정선성추세변화（F＝27．480，P＜0．05；F＝23．959，P＜0．05）。uTSH/uRBP적함량재각임신시단차이무통계학의의（F＝2．731，P＝0．054）。결론경uRBP교정후，건강잉부각임신시단uFT3화uFT4적함량정선성추세하강，uTSH적함량무명현변화추세。
Objective To further research the urinary monitoring of the maternal thyroid functional status ,the levels of urine thyroid hormones were analyzed during pregnancy .Methods This study recruited 30 cases of healthy pregnant women at 9-12 ges-tational weeks .Their random urine specimens were collected every four weeks until delivery .The concentrations of urine thyrotro-pin(uTSH) ,urine free triiodothyronine(uFT3) and urine free tetraiodothyronine(uFT4) were detected by electrochemical lumines-cence immunoassay(ECLIA) .The urine retinol binding protein(uRBP) was detected by enzyme immunoassay (EIA) .After correc-ted by uRBP ,the statistical analysis was done to analyze the variation of the ratio of uTSH and uRBP (uTSH/uRBP) ,the ratio of uFT3 and uRBP(uFT3/uRBP)and the ratio of uFT4 and uRBP(uFT4/uRBP)during pregnancy .Results The median levels of u-rine thyroid hormone parameters were obtained from the healthy pregnant women every four weeks and were compared among dif-ferent stages .The difference of uFT3/uRBP and uFT4/uRBP was statistically significant (F= 6 .222 ,P< 0 .05 ;F= 5 .078 ,P<0 .05) ,and the levels of them varied linearity during pregnancy (F=27 .480 ,P<0 .05 ;F=23 .959 ,P<0 .05) .The difference of uT-SH/uRBP had no statistical significance(F=2 .731 ,P=0 .054) .Conclusion After corrected by uRBP ,the levels of uFT3 and uFT4 of healthy pregnant women decreased linearity among different gestational stages ,and the levels of uTSH had no noticeable change .
