重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
22期
2841-2844
,共4页
张春燕%冯春红%敬健雄%段春燕%刘友平%夏先明%李洪%代荣阳%陈绍坤
張春燕%馮春紅%敬健雄%段春燕%劉友平%夏先明%李洪%代榮暘%陳紹坤
장춘연%풍춘홍%경건웅%단춘연%류우평%하선명%리홍%대영양%진소곤
细胞空泡%p38丝裂原活化蛋白激酶%内质网%SB203580
細胞空泡%p38絲裂原活化蛋白激酶%內質網%SB203580
세포공포%p38사렬원활화단백격매%내질망%SB203580
cytoplasmic vacuoles%p38 MAPK%endoplasmic reticulum%SB203580
目的：探讨p38丝裂原活化蛋白激酶（p38MAPK）通路与细胞空泡形成的关系。方法应用茴香霉素、放线菌酮、p38MAPK抑制剂SB203580、JNK抑制剂SP600125处理HepG2、LM3、QBC939、Hela和A549细胞，光学显微镜和激光共聚焦显微镜观察细胞空泡化情况；Westernblot法检测p38MAPK等通路相关分子的表达水平；内质网红色荧光探针标记内质网，激光共聚焦显微镜观察内质网结构变化；溶酶体红色荧光探针标记溶酶体，激光共聚焦显微镜观察溶酶体荧光染色情况。结果（1）茴香霉素对HepG2细胞空泡有消除作用。（2）茴香霉素通过活化p38MAPK消除细胞空泡。（3）阻断p38MAPK诱导多种肿瘤细胞空泡形成。（4）阻断p38MAPK介导的空泡形成破坏内质网结构的整体性。（5）阻断p38MAPK介导的空泡形成具有可逆性。结论p38MAPK通路在调节细胞空泡形成中发挥了重要作用。
目的：探討p38絲裂原活化蛋白激酶（p38MAPK）通路與細胞空泡形成的關繫。方法應用茴香黴素、放線菌酮、p38MAPK抑製劑SB203580、JNK抑製劑SP600125處理HepG2、LM3、QBC939、Hela和A549細胞，光學顯微鏡和激光共聚焦顯微鏡觀察細胞空泡化情況；Westernblot法檢測p38MAPK等通路相關分子的錶達水平；內質網紅色熒光探針標記內質網，激光共聚焦顯微鏡觀察內質網結構變化；溶酶體紅色熒光探針標記溶酶體，激光共聚焦顯微鏡觀察溶酶體熒光染色情況。結果（1）茴香黴素對HepG2細胞空泡有消除作用。（2）茴香黴素通過活化p38MAPK消除細胞空泡。（3）阻斷p38MAPK誘導多種腫瘤細胞空泡形成。（4）阻斷p38MAPK介導的空泡形成破壞內質網結構的整體性。（5）阻斷p38MAPK介導的空泡形成具有可逆性。結論p38MAPK通路在調節細胞空泡形成中髮揮瞭重要作用。
목적：탐토p38사렬원활화단백격매（p38MAPK）통로여세포공포형성적관계。방법응용회향매소、방선균동、p38MAPK억제제SB203580、JNK억제제SP600125처리HepG2、LM3、QBC939、Hela화A549세포，광학현미경화격광공취초현미경관찰세포공포화정황；Westernblot법검측p38MAPK등통로상관분자적표체수평；내질망홍색형광탐침표기내질망，격광공취초현미경관찰내질망결구변화；용매체홍색형광탐침표기용매체，격광공취초현미경관찰용매체형광염색정황。결과（1）회향매소대HepG2세포공포유소제작용。（2）회향매소통과활화p38MAPK소제세포공포。（3）조단p38MAPK유도다충종류세포공포형성。（4）조단p38MAPK개도적공포형성파배내질망결구적정체성。（5）조단p38MAPK개도적공포형성구유가역성。결론p38MAPK통로재조절세포공포형성중발휘료중요작용。
Objective To investigate the role of the p38 MAPK pathway in the formation of cytoplasmic vacuoles .Methods Af-ter treated with Anisomycin ,SB203580 or SP600125 ,images of HepG2 ,LM3 ,QBC939 ,Hela and A549 cells were recorded by light microscopy and taken at a magnification of 400 × .The effects of anisomycin ,SB203580 and SP600125 on the activity of p38 and JNK were measured by Western blot .LM3 and A549 cells were stained with the ER-tracker red and the lyso-tracker red and subjec-ted to confocal microscopy analysis .Results (1)Anisomycin could abolish cytoplasmic vacuolization of HepG2 cells .(2)p38 MAPK activation was responsible for anisomycin-induced cytoplasmic vacuolization abolishment .(3)p38 MAPK blocking initiated cytoplas-mic vacuoles formation in various cancer cell lines .(4)p38 MAPK blocking-induced cytoplasmic vacuoles disrupted the integrity of endoplasmic reticulum .(5)p38 MAPK blocking reversibly induced cytoplasmic vacuoles formation .Conclusion These observations provide direct evidence for a role of p38 MAPK signaling in regulating the formation of cytoplasmic vacuoles .
