CAJ | 학술논문

目的：观察小干扰RNA（siRNA）沉默STMN1其对食管癌Eca-109细胞紫杉醇敏感性的影响。方法将STMN1 siRNA通过瞬时转染于食管癌Eca-109细胞，使用qPCR和Western blot实验检验转染效果，通过M T T实验和平板克隆实验观察Eca-109细胞对紫杉醇敏感性的变化，并且通过 Hoechst 33258染料核染色后观察Eca-109细胞在紫杉醇作用下凋亡反应的差异。结果成功转染STMN1 siRNA并有效抑制了STMN1的表达。转染STMN1 siRNA后Eca-109细胞对紫杉醇的药物敏感性明显增加（P＜0．01），在紫杉醇作用下凋亡细胞增多（P＜0．01）。结论通过siRNA抑制STMN1的表达后可以增加食管癌Eca-109细胞对紫杉醇的药物敏感性。
목적：관찰소간우RNA（siRNA）침묵STMN1기대식관암Eca-109세포자삼순민감성적영향。방법장STMN1 siRNA통과순시전염우식관암Eca-109세포，사용qPCR화Western blot실험검험전염효과，통과M T T실험화평판극륭실험관찰Eca-109세포대자삼순민감성적변화，병차통과 Hoechst 33258염료핵염색후관찰Eca-109세포재자삼순작용하조망반응적차이。결과성공전염STMN1 siRNA병유효억제료STMN1적표체。전염STMN1 siRNA후Eca-109세포대자삼순적약물민감성명현증가（P＜0．01），재자삼순작용하조망세포증다（P＜0．01）。결론통과siRNA억제STMN1적표체후가이증가식관암Eca-109세포대자삼순적약물민감성。
Objective To investigate the effects of silencing STMN1 by siRNA on the sensitivity of oesophageal cancer cells Eca-109 to paclitaxel .Methods The STMN1 siRNA(siSTMN1) or scramble siRNA(SCR) were transient transfected into Eca-109 cells .The mRNA and protein levels of STMN1 were detected by qPCR and Western blot in the Eca-109 cells of different groups .In vitro paclitaxel sensitivity of siSTMN1 and SCR transfected Eca-109 cell lines was tested by MTT assay and colony formation as-say .Hoechst 33258 nuclear staining were used to investigate the effect of silencing STMN 1 on the sensitivity of SCR ,siSTMN1 transfected Eca-109 cells and nontreated counterparts under paclitaxel induced apoptosis .Results The transient transfection cell lines were successfully established .Both protein and mRNA levels of STMN1 were effectively down-regulated in the siSTMN1 transfected Eca-109 cells .Down-regulation of STMN1 significantly enhanced the sensitivity of Eca-109 cells in response to paclitaxel (P<0 .01) .In addition ,the siSTMN1 transfected Eca-109 cells displayed significant apoptosis as assessed by Hoechst nuclear stai-ning(P<0 .01) .Conclusion Silencing STMN1 by siRNA could enhance the sensitivity of oesophageal cancer cells Eca-109 to paclitaxel .