CAJ | 학술논문

目的：检测miR-133 b在帕金森病（ PD）患者脑脊液中的表达情况，为帕金森病的早期诊断提供参考依据。方法采用实时荧光定量RT-PCR检测46例PD患者、25例头痛患者和22例健康人脑脊液中miR-133b表达水平。分析脑脊液中miR-133b表达水平与PD相关临床指标的关系。结果 PD组患者脑脊液中miR-133 b相对表达水平明显高于头痛组和健康对照组（P均＜0．05），且PD患者脑脊液中miR-133b表达与帕金森综合评分量表（UPDRS）分级呈正相关（r＝0．74，P＜0．05）。 ROC曲线确诊PD患者的脑脊液miR-133b临界值为0．672，其诊断敏感度为87．7％，特异度为79．3％。结论脑脊液miR-133b的检测不仅有助于PD的诊断，也可能作为判断PD病情的指标之一。
목적：검측miR-133 b재파금삼병（ PD）환자뇌척액중적표체정황，위파금삼병적조기진단제공삼고의거。방법채용실시형광정량RT-PCR검측46례PD환자、25례두통환자화22례건강인뇌척액중miR-133b표체수평。분석뇌척액중miR-133b표체수평여PD상관림상지표적관계。결과 PD조환자뇌척액중miR-133 b상대표체수평명현고우두통조화건강대조조（P균＜0．05），차PD환자뇌척액중miR-133b표체여파금삼종합평분량표（UPDRS）분급정정상관（r＝0．74，P＜0．05）。 ROC곡선학진PD환자적뇌척액miR-133b림계치위0．672，기진단민감도위87．7％，특이도위79．3％。결론뇌척액miR-133b적검측불부유조우PD적진단，야가능작위판단PD병정적지표지일。
Objective It is to detect the expression of miR -133 b in cerebrospinal fluid of patients with Parkinson ’ s dis-ease ( PD) , so as to provide a reference on early diagnosis of PD .Methods The expression levels of miR -133 b in cerebro-spinal fluid of 46 patients with PD , 25 patients with headache and 22 healthy people were detected by Reverse transcription polymerase chain reaction (RT-PCR).The correlations of miR-133b expression and the clinicopathological features of PD were analyzed.Results The expression level of miR -133b in cerebrospinal fluid of patients with PD was significantly higher than headache group and healthy control group (all P<0.05).The expression level of miR -133b in cerebrospinal fluid had a positive correlation with Unified Parkinson ’s Disease Rating Scale (UPDRS) (r=0.74, P<0.05).The critical values for miR-133b in cerebrospinal fluid were detected at 0.672 through the ROC curves.Under the critical value, the sensitivity of miR-133b was 87.7%and the specificity was 79.3%.Conclusion The detection of miR -133b in cerebrospinal fluid not only contributes to the diagnosis of PD , but also be as an index to judge the condition of PD .