CAJ | 학술논문

目的：研究白藜芦醇对TRAIL诱导肝癌细胞SMMC-7721凋亡的影响及其机制。方法培养人肝癌细胞株SMMC-7721，分为对照组、TRAIL干预组、Res ＋TRAIL干预组，进行DNA末端原位标记（ TUNEL ）染色法作凋亡分析；Western Blot 检测白藜芦醇对SMMC-7721细胞 Survivn 表达的影响。结果对照组SMMC -7721细胞的凋亡指数为（1.78±04．9）％，TRAIL 干预组凋亡指数为（9．86±0．53）％，Res＋TRAIL干预组中25μmol/L、50μmol/L、100μmol/L浓度Ros凋亡指数分别为（11．30±0．80）％，（20．89±0．69）％和（27．78±1．88）％。除Res ＋TRAIL干预组（25μmol/L）与TRAIL干预组比较无显著性差异（P＞0．05），其余各组之间比较差异均有统计学意义（P均＜0．05）。 Western Blot测定结果显示：25μmol/L Ros处理组Survivin /β-actin的值与对照组相比无统计学差异（P＞0．05）；50μmol/L和100μmol/L Res处理组Survivin /β-actin的值较对照组显著降低（P＜0．01）；100μmol/L Res处理组Survivin /β-actin的值较50μmol/L显著降低（P＜0．01）。结论 TRAIL对SMMC-7721细胞有凋亡诱导作用；Res对TRAIL诱导人肝癌细胞SMMC-7721凋亡有致敏作用，并且这种作用呈剂量依赖关系；其分子机制可能与下调凋亡抑制因子Survivin的表达有关。
목적：연구백려호순대TRAIL유도간암세포SMMC-7721조망적영향급기궤제。방법배양인간암세포주SMMC-7721，분위대조조、TRAIL간예조、Res ＋TRAIL간예조，진행DNA말단원위표기（ TUNEL ）염색법작조망분석；Western Blot 검측백려호순대SMMC-7721세포 Survivn 표체적영향。결과대조조SMMC -7721세포적조망지수위（1.78±04．9）％，TRAIL 간예조조망지수위（9．86±0．53）％，Res＋TRAIL간예조중25μmol/L、50μmol/L、100μmol/L농도Ros조망지수분별위（11．30±0．80）％，（20．89±0．69）％화（27．78±1．88）％。제Res ＋TRAIL간예조（25μmol/L）여TRAIL간예조비교무현저성차이（P＞0．05），기여각조지간비교차이균유통계학의의（P균＜0．05）。 Western Blot측정결과현시：25μmol/L Ros처리조Survivin /β-actin적치여대조조상비무통계학차이（P＞0．05）；50μmol/L화100μmol/L Res처리조Survivin /β-actin적치교대조조현저강저（P＜0．01）；100μmol/L Res처리조Survivin /β-actin적치교50μmol/L현저강저（P＜0．01）。결론 TRAIL대SMMC-7721세포유조망유도작용；Res대TRAIL유도인간암세포SMMC-7721조망유치민작용，병차저충작용정제량의뢰관계；기분자궤제가능여하조조망억제인자Survivin적표체유관。
Objective It is to observe the influence of resveratrol on TRAIL -induced apoptosis in human hepatoma cell line SMMC-7721 and its mechanism .Methods SMMC-7721 cells were cultured in vitro and divided into three gropus:con-trol group, TRAIL intervention group, resveratrol and TRAIL intervention group .After treatment with TRAIL/resveratrol and TRAIL for 24 hours, TUNEL staining were given to detect the effects of resveratrol on TRAIL -induced apoptosis in human hepatoma cell line SMMC -7721;Western blot technology was performed to detect the expression of apoptosis -related protein survivin in hepatoma apoptotic cells treated by resveratrol .Results The apoptotic rate of SMMC-7721 cells was (1.78 ±0. 49)%in control group, and was (9.86 ±0.53)% in TRAIL intervention group, was (11.30 ±0.80)%, (20.89 ±0. 69)%, (27.78 ±1.88)%respectively after cotreatment with 100 μg/L TRAIL and 25 μmol/L or 50 μmol/L or 100 μmol/L resveratrol for 24 hours in resveratrol and TRAIL intervention group .There was significant difference between every two groups, excepted 25 μmol/L resveratrol intervention group with TRAIL intervention group (P<0.05).The results of detec-tion with Western Blot technology indicated that after being treated by 50 μmol/L, 100 μmol/L resveratrol for 24 hours, the value of surviving/β-actin was down-regulated significantly compared with control group (P<0.01);and there was signifi-cant difference between 50 μmol/L and 100 μmol/L resveratrol-treated group (P<0.01).Conclusion TRAIL can induce apoptosis of human hepatoma SMMC -7721 cells in vitro.Resveratrol can enhance TRAIL -induced apoptosis of SMMC -7721 cells in dose-dependent manner .This enhancement may be related to the inhibition of expression of survivin by resveratrol .