CAJ | 학술논문

目的：探讨miR-21和miR-93联合检测在三阴性乳腺癌（ TNBC）中的表达情况及与临床病理特征的关系。方法选取行乳腺癌根治的TNBC患者33例，选取同期正常乳腺组织15例。采用RT-PCR方法检测miR-21和miR-93在TNBC组织和正常乳腺组织中的相对表达量。结果 miR-21和miR-93在TNBC组织中的相对表达量分别为0.874±0．124和0．764±0．163，而在正常乳腺组织的表达量分别为0．235±0．034和0．183±0．028，TNBC组织中的miR-21和miR-93表达量明显高于正常乳腺组织（P均＜0.01）。miR-21和miR-93基因的高表达与年龄、月经状态无明显相关性，而与肿瘤直径、临床分期和淋巴结转移具有明显的相关性（P＜0．05或0．01）。组织中miR-21和miR-93的高表达呈正相关（r＝0．732，P＜0．01）。结论 miR-21和miR-93参与了TNBC的发生、发展和转移，是重要分子标记物。
목적：탐토miR-21화miR-93연합검측재삼음성유선암（ TNBC）중적표체정황급여림상병리특정적관계。방법선취행유선암근치적TNBC환자33례，선취동기정상유선조직15례。채용RT-PCR방법검측miR-21화miR-93재TNBC조직화정상유선조직중적상대표체량。결과 miR-21화miR-93재TNBC조직중적상대표체량분별위0.874±0．124화0．764±0．163，이재정상유선조직적표체량분별위0．235±0．034화0．183±0．028，TNBC조직중적miR-21화miR-93표체량명현고우정상유선조직（P균＜0.01）。miR-21화miR-93기인적고표체여년령、월경상태무명현상관성，이여종류직경、림상분기화림파결전이구유명현적상관성（P＜0．05혹0．01）。조직중miR-21화miR-93적고표체정정상관（r＝0．732，P＜0．01）。결론 miR-21화miR-93삼여료TNBC적발생、발전화전이，시중요분자표기물。
Objective It is to observe the expression of miR -21 and miR-93 in triple negative breast cancer ( TNBC) and their relationship with clinicopathological features .Methods 33 patients with TNBC treated with radical mastectomy and 15 cases with normal breast tissue were selected .The relative expression levels of miR-21 and miR-93 were detected by RT-PCR in TNBC tissue and normal breast tissue .Results The relative gene expression of miR -21 and miR-93 in TNBC tissue were 0.874 ±0.124 and 0.764 ±0.163 respectively, while were 0.235 ±0.034 and 0.183 ±0.028 respectively in normal breast tissue , the expression of miR-21 and miR-93 in TNBC were significantly higher than that in normal breast tissue ( P<0.01).The high expressions of miR -21 and miR-93 were not related to age and menstrual status , but obviously related with the tumor size, clinical stage, lymph node metastasis(P<0.05 or 0.01).The high expression of miR -21 in TNBC was positively correlated to the expression of miR -93 (r=0.732, P<0.01).Conclusion The gene of miR-21 and miR-93 are involved in the occurrence , development and metastasis of TNBC , and they are important molecular markers .