中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
33期
5317-5322
,共6页
奉有才%邓耀良%陶芝伟%王翔%黎承扬%黄鹏%吴博
奉有纔%鄧耀良%陶芝偉%王翔%黎承颺%黃鵬%吳博
봉유재%산요량%도지위%왕상%려승양%황붕%오박
组织构建%组织工程%高迁移率族蛋白B1%炎症因子%巨噬细胞%协同作用%细胞培养技术%磷酸钙晶体%炎症%肾结石%国家自然科学基金
組織構建%組織工程%高遷移率族蛋白B1%炎癥因子%巨噬細胞%協同作用%細胞培養技術%燐痠鈣晶體%炎癥%腎結石%國傢自然科學基金
조직구건%조직공정%고천이솔족단백B1%염증인자%거서세포%협동작용%세포배양기술%린산개정체%염증%신결석%국가자연과학기금
kidney calculi%high mobility group proteins%interleukin-1beta%interleukin-6%tumor necrosis factor-alpha%macrophages
背景：研究表明，巨噬细胞及其炎症反应参与了肾结石的发生发展。前期实验发现结石晶体可刺激巨噬细胞释放高迁移率族蛋白B1。<br> 目的：观察高迁移率族蛋白B1对磷酸钙诱导巨噬细胞释放白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化因子1的协同作用。<br> 方法：实验分两部分：①将成功诱导为巨噬细胞的U937细胞分为空白组、100 mg/L磷酸钙组、100μg/L高迁移率族蛋白B1组、100 mg/L磷酸钙+100μg/L高迁移率族蛋白B1组，干预1，2，4 h后收集细胞上清液。②将已成功诱导为巨噬细胞的U937细胞分为100 mg/L磷酸钙组、磷酸钙+10μg/L高迁移率族蛋白B1组、磷酸钙+50μg/L高迁移率族蛋白B1组、磷酸钙+100μg/L高迁移率族蛋白B1组，干预4 h后收集细胞上清液。Elisa法检测白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化因子1水平。<br> 结果与结论：ELISA结果显示，磷酸钙组，100μg/L高迁移率族蛋白B1组上清液白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化因子1质量浓度均高于空白组，磷酸钙+100μg/L高迁移率族蛋白B1组上清液上述因子质量浓度均显著高于其他3组(P<0.05)，且呈时间依赖性。不同质量浓度高迁移率族蛋白B1+磷酸钙组细胞上清液白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化因子1水平均显著高于磷酸钙组(P<0.05)，且呈浓度依赖性。结果表明，磷酸钙及高迁移率族蛋白B1均可诱导巨噬细胞释放白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化因子1；高迁移率族蛋白B1可协同磷酸钙诱导巨噬细胞释放白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化因子1。
揹景：研究錶明，巨噬細胞及其炎癥反應參與瞭腎結石的髮生髮展。前期實驗髮現結石晶體可刺激巨噬細胞釋放高遷移率族蛋白B1。<br> 目的：觀察高遷移率族蛋白B1對燐痠鈣誘導巨噬細胞釋放白細胞介素1β、白細胞介素6、腫瘤壞死因子α、單覈細胞趨化因子1的協同作用。<br> 方法：實驗分兩部分：①將成功誘導為巨噬細胞的U937細胞分為空白組、100 mg/L燐痠鈣組、100μg/L高遷移率族蛋白B1組、100 mg/L燐痠鈣+100μg/L高遷移率族蛋白B1組，榦預1，2，4 h後收集細胞上清液。②將已成功誘導為巨噬細胞的U937細胞分為100 mg/L燐痠鈣組、燐痠鈣+10μg/L高遷移率族蛋白B1組、燐痠鈣+50μg/L高遷移率族蛋白B1組、燐痠鈣+100μg/L高遷移率族蛋白B1組，榦預4 h後收集細胞上清液。Elisa法檢測白細胞介素1β、白細胞介素6、腫瘤壞死因子α、單覈細胞趨化因子1水平。<br> 結果與結論：ELISA結果顯示，燐痠鈣組，100μg/L高遷移率族蛋白B1組上清液白細胞介素1β、白細胞介素6、腫瘤壞死因子α、單覈細胞趨化因子1質量濃度均高于空白組，燐痠鈣+100μg/L高遷移率族蛋白B1組上清液上述因子質量濃度均顯著高于其他3組(P<0.05)，且呈時間依賴性。不同質量濃度高遷移率族蛋白B1+燐痠鈣組細胞上清液白細胞介素1β、白細胞介素6、腫瘤壞死因子α、單覈細胞趨化因子1水平均顯著高于燐痠鈣組(P<0.05)，且呈濃度依賴性。結果錶明，燐痠鈣及高遷移率族蛋白B1均可誘導巨噬細胞釋放白細胞介素1β、白細胞介素6、腫瘤壞死因子α、單覈細胞趨化因子1；高遷移率族蛋白B1可協同燐痠鈣誘導巨噬細胞釋放白細胞介素1β、白細胞介素6、腫瘤壞死因子α、單覈細胞趨化因子1。
배경：연구표명，거서세포급기염증반응삼여료신결석적발생발전。전기실험발현결석정체가자격거서세포석방고천이솔족단백B1。<br> 목적：관찰고천이솔족단백B1대린산개유도거서세포석방백세포개소1β、백세포개소6、종류배사인자α、단핵세포추화인자1적협동작용。<br> 방법：실험분량부분：①장성공유도위거서세포적U937세포분위공백조、100 mg/L린산개조、100μg/L고천이솔족단백B1조、100 mg/L린산개+100μg/L고천이솔족단백B1조，간예1，2，4 h후수집세포상청액。②장이성공유도위거서세포적U937세포분위100 mg/L린산개조、린산개+10μg/L고천이솔족단백B1조、린산개+50μg/L고천이솔족단백B1조、린산개+100μg/L고천이솔족단백B1조，간예4 h후수집세포상청액。Elisa법검측백세포개소1β、백세포개소6、종류배사인자α、단핵세포추화인자1수평。<br> 결과여결론：ELISA결과현시，린산개조，100μg/L고천이솔족단백B1조상청액백세포개소1β、백세포개소6、종류배사인자α、단핵세포추화인자1질량농도균고우공백조，린산개+100μg/L고천이솔족단백B1조상청액상술인자질량농도균현저고우기타3조(P<0.05)，차정시간의뢰성。불동질량농도고천이솔족단백B1+린산개조세포상청액백세포개소1β、백세포개소6、종류배사인자α、단핵세포추화인자1수평균현저고우린산개조(P<0.05)，차정농도의뢰성。결과표명，린산개급고천이솔족단백B1균가유도거서세포석방백세포개소1β、백세포개소6、종류배사인자α、단핵세포추화인자1；고천이솔족단백B1가협동린산개유도거서세포석방백세포개소1β、백세포개소6、종류배사인자α、단핵세포추화인자1。
BACKGROUND:More and more evidence suggests that macrophages and inflammation reactions are involved in the formation and development of nephrolithiasis. Previous studies have found that calculi crystals can stimulate macrophages to release high mobility group protein B1. <br> OBJECTIVE:To investigate the synergistic effect of high mobility group protein B1 in calcium phosphate induced release of interleukin-1β, interleukin-6, tumor necrosis factorαand monocyte chemotactic factor 1 from human macrophages. <br> METHODS:(1) The induced U937 cells were respectively stimulated with RPMI (blank), 100 mg/L calcium phosphate, 100μg/L high mobility group protein B1 and 100 mg/L calcium phosphate+100μg/L high mobility group protein B1 for 1, 2 and 4 hours to col ect cellsupernatant. (2) The induced U937 cells were respectively stimulated with 100 mg/L calcium phosphate, 100 mg/L calcium phosphate+10μg/L high mobility group protein B1, 100 mg/L calcium phosphate+50μg/L high mobility group protein B1, 100 mg/L calcium phosphate+100μg/L high mobility group protein B1 for 4 hours to col ect cellsupernatant. Levels of interleukin-1β, interleukin-6, tumor necrosis factorαand monocyte chemotactic factor 1 were determined by ELISA. <br> RESULTS AND CONCLUSION:The levels of interleukin-1β, interleukin-6, tumor necrosis factorαand monocyte chemotactic factor 1 in the cellculture supernatant of 100 mg/L calcium phosphate group and 100μg/L high mobility group protein B1 group were both higher than those in the blank group in a time-dependent manner (P<0.05). The levels of interleukin-1β, interleukin-6, tumor necrosis factorαand monocyte chemotactic factor 1 in the cellculture supernatant of different concentrations of high mobility group protein B1 groups were al higher than those in the 100 mg/L calcium phosphate group in a concentration-dependent manner (P<0.05). The results suggest that both calcium phosphate and high mobility group protein B1 can induce the release of interleukin-1β, interleukin-6, tumor necrosis factorαand monocyte chemotactic factor 1 from human macrophages and the high mobility group protein B1 has the synergistic effect with calcium phosphate to induce interleukin-1β, interleukin-6, tumor necrosis factorαand monocyte chemotactic factor 1 from human macrophages.
