CAJ | 학술논문

背景：既往研究发现接合性质粒pRST98可促进其宿主菌生物膜的形成，携带pRST98的菌株感染细胞和实验动物后，可促进白细胞介素10的分泌和表达。 　　目的：体外研究不同质量浓度白细胞介素10与接合性质粒pRST98对鼠伤寒沙门菌生物膜形成的相互影响。 　　方法：体外建立鼠伤寒沙门菌标准株χ3306、突变株χ3337及接合株χ3337/pRST983组生物膜模型，3组分别加入0(空白对照)，1，10，100μg/L白细胞介素10，通过结晶紫染色半定量法、共聚焦激光扫描显微镜分析和扫描电镜观察，比较不同质量浓度白细胞介素10对携带和不携带接合性质粒 pRST98的鼠伤寒沙门菌生物膜形成的影响。 　　结果与结论：①3组组内比较：与空白对照组比较，1，10μg/L白细胞介素10均能促进鼠伤寒沙门菌的聚集，提高生物膜形成能力，且10μg/L质量浓度效果更明显；100μg/L白细胞介素10抑制鼠伤寒沙门菌生物膜的形成。②3组组间比较：在同一白细胞介素10质量浓度下，接合株χ3337/pRST98组 A570高于标准株χ3306组、突变株χ3337组。结果说明1，10μg/L白细胞介素10可促进生物膜的形成，且在携带接合性质粒pRST98的情况下促进作用更明显。
배경：기왕연구발현접합성질립pRST98가촉진기숙주균생물막적형성，휴대pRST98적균주감염세포화실험동물후，가촉진백세포개소10적분비화표체。 　　목적：체외연구불동질량농도백세포개소10여접합성질립pRST98대서상한사문균생물막형성적상호영향。 　　방법：체외건립서상한사문균표준주χ3306、돌변주χ3337급접합주χ3337/pRST983조생물막모형，3조분별가입0(공백대조)，1，10，100μg/L백세포개소10，통과결정자염색반정량법、공취초격광소묘현미경분석화소묘전경관찰，비교불동질량농도백세포개소10대휴대화불휴대접합성질립 pRST98적서상한사문균생물막형성적영향。 　　결과여결론：①3조조내비교：여공백대조조비교，1，10μg/L백세포개소10균능촉진서상한사문균적취집，제고생물막형성능력，차10μg/L질량농도효과경명현；100μg/L백세포개소10억제서상한사문균생물막적형성。②3조조간비교：재동일백세포개소10질량농도하，접합주χ3337/pRST98조 A570고우표준주χ3306조、돌변주χ3337조。결과설명1，10μg/L백세포개소10가촉진생물막적형성，차재휴대접합성질립pRST98적정황하촉진작용경명현。
BACKGROUND:Previous studies discovered that pRST98, original y isolated from Salmonel a enterica serovar typhimurium (S.typhimurium) could promote bacterial biofilm formation. In addition, bacterial harboring pRST98 can promote the secretion and expression of interleukin-10 after infection in cells and animals. OBJECTIVE:In vitro studies have discovered the effects of interleukin-10 at varying concentrations and conjugative plasmid pRST98 on the biofilm formation of S.typhimurium. METHODS:S.typhimurium wild-type strainχ3306, virulence plasmid-deletion S.typhimurium strainχ3337 and pRST98-transconjugant S.typhimuriumχ3337/pRST98 were established in vitro and cultured for biofilm formation. 1, 10, 100 μg/L interleukin-10 were added during the biofilm formation. 0 μg/L interleukin-10 was set as a control. Crystal violet staining method, semi-quantitative method, confocal laser scanning microscopy and scanning electron microscopy were used to determine the effects of interleukin-10 on the biofilm formation and compare the effects of S.typhimurium with or without pRST98. RESULTS AND CONCLUSION:Intra-group comparison showed that, compared with the control group, S.typhimurium gathered together and formed thicker biofilm in concentration of 1 and 10 μg/L of interleukin-10. The promotion effects of S.typhimurium on biofilm formation were greatly improved in 10 μg/L. Interleukin-10 in 100 μg/L inhibited S.typhimurium biofilm formation. Inter-group comparison showed that, A570 inχ3337/pRST98 was greatly higher than that inχ3306 andχ3337 under the same concentration of interleukin-10. The results indicate that both 1 and 10 μg/L of interleukin-10 promote biofilm formation, especial y bacteria harboring pRST98.