CAJ | 학술논문

背景：前期研究发现将膨体聚四氟乙烯单瓣同时植入绵羊降主动脉和左肺动脉20周，无明显血栓、钙化或降解，验证了其优良的特性，但在左肺动脉处有明显的新生内膜增生。 　　目的：观察磷酰胆碱表面改性超微孔膨体聚四氟乙烯的生物相容性变化。 　　方法：以磷酰胆碱对膨体聚四氟乙烯进行表面改性，观察改性前后膨体聚四氟乙烯表面形状、接触角、屈服点拉伸应力及弹性模量、蛋白吸附能力的变化。①溶血实验：在稀释人血中分别加入改性膨体聚四氟乙烯浸提液、膨体聚四氟乙烯浸提液、生理盐水及蒸馏水。②血小板计数实验：在健康人全血中分别加入改性膨体聚四氟乙烯、膨体聚四氟乙烯、高密度聚乙烯及酵母聚糖 A。③血小板激活实验：在健康人全血中分别加入改性膨体聚四氟乙烯、膨体聚四氟乙烯、γ-球蛋白及酵母聚糖A。 　　结果与结论：与改性前比较，改性后膨体聚四氟乙烯的微孔面积显著减小(P <0.001)，亲水性及抑制蛋白吸附能力显著增强(P <0.001)，生物力学性能及溶血性能无明显变化。体外血小板计数和血小板激活实验显示，改性后膨体聚四氟乙烯的抗血栓性能显著增强。说明磷酰胆碱表面改性可以增强超微孔膨体聚四氟乙烯的抗血栓性和抗蛋白吸附能力，改善其生物相容性。
배경：전기연구발현장팽체취사불을희단판동시식입면양강주동맥화좌폐동맥20주，무명현혈전、개화혹강해，험증료기우량적특성，단재좌폐동맥처유명현적신생내막증생。 　　목적：관찰린선담감표면개성초미공팽체취사불을희적생물상용성변화。 　　방법：이린선담감대팽체취사불을희진행표면개성，관찰개성전후팽체취사불을희표면형상、접촉각、굴복점랍신응력급탄성모량、단백흡부능력적변화。①용혈실험：재희석인혈중분별가입개성팽체취사불을희침제액、팽체취사불을희침제액、생리염수급증류수。②혈소판계수실험：재건강인전혈중분별가입개성팽체취사불을희、팽체취사불을희、고밀도취을희급효모취당 A。③혈소판격활실험：재건강인전혈중분별가입개성팽체취사불을희、팽체취사불을희、γ-구단백급효모취당A。 　　결과여결론：여개성전비교，개성후팽체취사불을희적미공면적현저감소(P <0.001)，친수성급억제단백흡부능력현저증강(P <0.001)，생물역학성능급용혈성능무명현변화。체외혈소판계수화혈소판격활실험현시，개성후팽체취사불을희적항혈전성능현저증강。설명린선담감표면개성가이증강초미공팽체취사불을희적항혈전성화항단백흡부능력，개선기생물상용성。
BACKGROUND:Our preliminary study found that the monocusp valves made of ultramicropore expanded polytetrafluoroethylene (ePTFE) revealed no significant thrombus, calcification, or degradation 20 weeks after implanted into the descending aorta and the left pulmonary artery in sheep, which verified the good property of ePTFE. However, the surface of ePTFE in the left pulmonary artery was covered with obvious neointima. OBJECTIVE: To assess the biocompatibility of phosphorylcholine-coated ePTFE. METHODS:ePTFE surface was modified by phosphorylcholine derivative. Then the changes of surface shape, tensile stress at yield and elasticity modulus, water contact angle, and protein absorption capacity of ePTFE after surface modification were observed. (1) Hemolytic test: the leaching solution of phosphorylcholine-coated ePTFE, leaching solution of uncoated ePTFE, normal saline, and distiled water were added to the diluted human blood, respectively. (2) Platelet count test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, high density polyethylene, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively. (3) Platelet activation test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, γ-Globulins, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively. RESULTS AND CONCLUSION: The mean micropore diameter of ePTFE was significantly decreased after phosphorylcholine coating (P < 0.001). The hydrophilicity and the ability of suppressing protein adsorption were significantly strengthened after phosphorylcholine coating (P < 0.001). Phosphorylcholine coating did not influence ePTFE in biomechanical properties and hemolytic test. The platelet count test and platelet activation test demonstrated that phosphorylcholine coating significantly improved anti-thrombus function of ePTFE. So, phosphorylcholine coating can enhance anti-thrombus function, suppress protein adsorption, and improve biocompatibility of ePTFE.