CAJ | 학술논문

背景：尽管温敏性壳聚糖是壳聚糖的一种，但其止血效果、组织相容性及体内代谢吸收情况还需要验证。目的：探讨温敏性壳聚糖止血膜的止血作用、体内降解和组织相容性。 　　方法：取SD大鼠48只，随机均分为4组，同时进行两种实验：①制作肝脏创面出血模型，其中3组分别采用温敏性壳聚糖止血膜、纤维素止血棉和明胶海绵止血材料贴敷大鼠肝创面止血，以不做任何处理的为空白对照。记录出血时间及出血量。②在上述3组大鼠的股四头肌内分别再植入对应的止血材料，空白对照组不植入任何材料。术后1，2，3，4，6周取两处创面行大体观察，术后4周行苏木精-伊红染色和电镜观察。结果与结论：温敏性壳聚糖组、纤维素组止血时间和出血量少于空白对照组、明胶海绵组(P <0.05)。温敏性壳聚糖止血膜于术后6周完全降解，纤维素止血棉于术后3周完全降解，明胶海绵于术后2周完全降解。温敏性壳聚糖组肝小叶结构完整，肝细胞结构基本正常，肿胀轻，炎症细胞浸润程度轻，电镜显示整个肝细胞外形结构清楚，细胞核无破损，细胞器良好；肌肉纤维结构完整，炎症细胞浸润程度轻，电镜显示肌纤维走向整齐，肌细胞核形态正常，细胞器良好。可见温敏性壳聚糖止血膜具有较好的止血作用和组织相容性。
배경：진관온민성각취당시각취당적일충，단기지혈효과、조직상용성급체내대사흡수정황환수요험증。목적：탐토온민성각취당지혈막적지혈작용、체내강해화조직상용성。 　　방법：취SD대서48지，수궤균분위4조，동시진행량충실험：①제작간장창면출혈모형，기중3조분별채용온민성각취당지혈막、섬유소지혈면화명효해면지혈재료첩부대서간창면지혈，이불주임하처리적위공백대조。기록출혈시간급출혈량。②재상술3조대서적고사두기내분별재식입대응적지혈재료，공백대조조불식입임하재료。술후1，2，3，4，6주취량처창면행대체관찰，술후4주행소목정-이홍염색화전경관찰。결과여결론：온민성각취당조、섬유소조지혈시간화출혈량소우공백대조조、명효해면조(P <0.05)。온민성각취당지혈막우술후6주완전강해，섬유소지혈면우술후3주완전강해，명효해면우술후2주완전강해。온민성각취당조간소협결구완정，간세포결구기본정상，종창경，염증세포침윤정도경，전경현시정개간세포외형결구청초，세포핵무파손，세포기량호；기육섬유결구완정，염증세포침윤정도경，전경현시기섬유주향정제，기세포핵형태정상，세포기량호。가견온민성각취당지혈막구유교호적지혈작용화조직상용성。
BACKGROUND:The thermosensitive chitosan is a kind of chitosan, its hemostatic effect, tissue compatibility andin vivo absorption need further investigations. OBJECTIVE:To investigate the hemostasis,in vivo degradation and tissue compatibility of thermosensitive chitosan hemostatic film. METHODS: A total of 48 Sprague-Dawley rats were randomly divided into four groups, and carried out two experiments at the same time. (1) The incisions of the liver in three groups were covered with the thermosensitive chitosan hemostatic film, celulose hemostatic cotton and gelatin sponge, respectively. Blank control group received no treatment. The bleeding time and bleeding amount were recorded. (2) The incisions of the quadriceps femoris muscle of rats in the above three groups were embedded with the same hemostatic materials respectively. Blank control group was not embedded. At 1, 2, 3, 4, 6 weeks, the incision tissues of the liver and the quadriceps femoris muscle were harvested for observation. After 4 weeks, the incisions were observed with hematoxylin- eosin staining and transmission electron microscopy. RESULTS AND CONCLUSION: The bleeding time and bleeding amount of thermosensitive chitosan hemostatic film and celulose hemostatic cotton groups were significantly lower than those of gelatin sponge and blank control groups (P < 0.05). After 6 weeks, the thermosensitive chitosan hemostatic film was absorbed completely. After 3 weeks, the celulose hemostatic cotton was absorbed completely. After 2 weeks, the gelatin sponge was absorbed completely. The liver lobules of thermosensitive chitosan hemostatic film were complete, the liver cellwere normal structure, showing light sweling and little inflammatory cellinfiltration. Under transmission electron microscopy, the liver cels had integral structure, cellnucleus and organeles remained intact. The muscle fibers showed complete structure and little inflammatory cellinfiltration. Under transmission electron microscopy, the muscle fibers ranked tidily, with integral cellnucleus and organeles. The thermosensitive chitosan hemostatic film has good hemostasis effect and tissue compatibility.