中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
34期
5466-5472
,共7页
康雯%徐国强%迪丽努尔阿吉%魏琴%马慧%李兆鹏%宋倩%茹菲娅祖拉提%阿丽娜阿布都吉力力%排黑尔丁凯赛尔
康雯%徐國彊%迪麗努爾阿吉%魏琴%馬慧%李兆鵬%宋倩%茹菲婭祖拉提%阿麗娜阿佈都吉力力%排黑爾丁凱賽爾
강문%서국강%적려노이아길%위금%마혜%리조붕%송천%여비아조랍제%아려나아포도길력력%배흑이정개새이
生物材料%材料相容性%钛酸钡%压电陶瓷%细胞毒性%生物相容性%CCK-8%等离子喷涂%骨髓间充质干细胞%羟基磷灰石%扫描电子显微镜%新疆维吾尔自治区自然科学基金
生物材料%材料相容性%鈦痠鋇%壓電陶瓷%細胞毒性%生物相容性%CCK-8%等離子噴塗%骨髓間充質榦細胞%羥基燐灰石%掃描電子顯微鏡%新疆維吾爾自治區自然科學基金
생물재료%재료상용성%태산패%압전도자%세포독성%생물상용성%CCK-8%등리자분도%골수간충질간세포%간기린회석%소묘전자현미경%신강유오이자치구자연과학기금
ceramics%materials testing%hydroxyapatites
背景:为优化羟基磷灰石的生物活性,前期实验利用等离子喷涂技术在羟基磷灰石表面等离子喷涂制备了压电陶瓷涂层,但此种新型材料的细胞毒性还不明确。<br> 目的:评价羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性。<br> 方法:将第3代比格犬骨髓间充质干细胞分别接种于羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件上,接种5 d后,扫描电镜观察材料上细胞黏附情况。分别以羟基磷灰石/钛酸钡压电陶瓷试件浸提液、羟基磷灰石试件浸提液、含5%二甲基亚砜和体积分数15%胎牛血清的低糖DMEM溶液、只含体积分数15%胎牛血清的低糖DMEM培养基培养第3代比格犬骨髓间充质干细胞,培养的1,3,5 d采用CCK-8法检测各组细胞毒性。结果与结论:骨髓间充质干细胞在羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件表面增殖旺盛,达到复层生长,伪足与细胞之间连接很紧密,胞体丰富,表明两组材料具有良好的细胞相容性。CCK-8细胞毒性实验显示,羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件组细胞相对增殖率均在80%以上,毒性分级为1级,无细胞毒性。
揹景:為優化羥基燐灰石的生物活性,前期實驗利用等離子噴塗技術在羥基燐灰石錶麵等離子噴塗製備瞭壓電陶瓷塗層,但此種新型材料的細胞毒性還不明確。<br> 目的:評價羥基燐灰石/鈦痠鋇壓電陶瓷塗層的體外細胞毒性。<br> 方法:將第3代比格犬骨髓間充質榦細胞分彆接種于羥基燐灰石/鈦痠鋇壓電陶瓷試件與羥基燐灰石試件上,接種5 d後,掃描電鏡觀察材料上細胞黏附情況。分彆以羥基燐灰石/鈦痠鋇壓電陶瓷試件浸提液、羥基燐灰石試件浸提液、含5%二甲基亞砜和體積分數15%胎牛血清的低糖DMEM溶液、隻含體積分數15%胎牛血清的低糖DMEM培養基培養第3代比格犬骨髓間充質榦細胞,培養的1,3,5 d採用CCK-8法檢測各組細胞毒性。結果與結論:骨髓間充質榦細胞在羥基燐灰石/鈦痠鋇壓電陶瓷試件與羥基燐灰石試件錶麵增殖旺盛,達到複層生長,偽足與細胞之間連接很緊密,胞體豐富,錶明兩組材料具有良好的細胞相容性。CCK-8細胞毒性實驗顯示,羥基燐灰石/鈦痠鋇壓電陶瓷試件與羥基燐灰石試件組細胞相對增殖率均在80%以上,毒性分級為1級,無細胞毒性。
배경:위우화간기린회석적생물활성,전기실험이용등리자분도기술재간기린회석표면등리자분도제비료압전도자도층,단차충신형재료적세포독성환불명학。<br> 목적:평개간기린회석/태산패압전도자도층적체외세포독성。<br> 방법:장제3대비격견골수간충질간세포분별접충우간기린회석/태산패압전도자시건여간기린회석시건상,접충5 d후,소묘전경관찰재료상세포점부정황。분별이간기린회석/태산패압전도자시건침제액、간기린회석시건침제액、함5%이갑기아풍화체적분수15%태우혈청적저당DMEM용액、지함체적분수15%태우혈청적저당DMEM배양기배양제3대비격견골수간충질간세포,배양적1,3,5 d채용CCK-8법검측각조세포독성。결과여결론:골수간충질간세포재간기린회석/태산패압전도자시건여간기린회석시건표면증식왕성,체도복층생장,위족여세포지간련접흔긴밀,포체봉부,표명량조재료구유량호적세포상용성。CCK-8세포독성실험현시,간기린회석/태산패압전도자시건여간기린회석시건조세포상대증식솔균재80%이상,독성분급위1급,무세포독성。
BACKGROUND:In order to optimize the biological activity of hydroxyapatite, previous experiments have used plasma spraying technique to prepare a piezoelectric ceramic coating on the surface of hydroxyapatite, but the cytotoxicity of this new material is not clear. <br> OBJECTIVE: To evaluate the cytotoxicity of hydroxyapatite/barium titanate biological piezoelectric ceramic coatingin vitro. <br> METHODS: The 3rd generation beagle bone marrow mesenchymal stem cels were seeded on <br> hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens, respectively. <br> After 5 days, the celladhesion was detected by scanning electron microscopy. The 3rd generation bone marrow <br> mesenchymal stem cels were also co-cultured with hydroxyapatite/barium titanate piezoelectric ceramic specimen extract, hydroxyapatite specimen extract, low-glucose Dulbecco’s modified Eagle’s medium containing 5% <br> dimethylsulfoxide and 15% fetal bovine serum, and low-glucose Dulbecco’s modified Eagle’s medium containing 15% <br> fetal bovine serum, respectively. The cytotoxicity was tested by cellCounting Kit-8 assay at days 1, 3, 5 after co-culture. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cels on the surface of hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens grew proliferatively and presented with multi-layer <br> growth. The connection between cels and pseudopodia was very close, which indicates that the two kinds of materials both have good cytocompatibility. cellCounting Kit-8 assay showed that the cels cultured in the extracts of <br> hydroxyapatite/barium titanate biological piezoelectric ceramic and hydroxyapatite specimens proliferated more than 80%, and the toxicity was grade 1 that meant no cytotoxicity.