CAJ | 학술논문

背景：京尼平的低毒性具有一定的种属和细胞特异性，人脂肪间充质干细胞与京尼平交联的Ⅰ型胶原蛋白支架材料的生物相容性对于应用两者构建组织工程脂肪至关重要。 　　目的：评估人脂肪间充质干细胞与京尼平交联的Ⅰ型胶原蛋白支架材料的生物相容性。 　　方法：分离培养人脂肪间充质干细胞，传代培养至第3代，接种于京尼平交联的Ⅰ型胶原蛋白支架材料上。MTT法评估人脂肪间充质干细胞在支架材料上的黏附和增殖情况，支架材料对细胞的毒性作用；光镜和电镜分别观察人脂肪间充质干细胞在支架材料上的黏附和生长过程，以及细胞形态学变化。 　　结果与结论：人脂肪间充质干细胞接种于支架材料后，能够迅速在材料上黏附、增殖，其黏附率平均为86.5%；光镜和扫描电镜显示人脂肪间充质干细胞在支架材料上黏附性良好，随着时间的推移，细胞逐渐增多，可以迁移进入支架内部并均匀分布。结果表明京尼平对细胞的毒性低，交联后的支架材料与人脂肪间充质干细胞具有良好的体外生物相容性。
배경：경니평적저독성구유일정적충속화세포특이성，인지방간충질간세포여경니평교련적Ⅰ형효원단백지가재료적생물상용성대우응용량자구건조직공정지방지관중요。 　　목적：평고인지방간충질간세포여경니평교련적Ⅰ형효원단백지가재료적생물상용성。 　　방법：분리배양인지방간충질간세포，전대배양지제3대，접충우경니평교련적Ⅰ형효원단백지가재료상。MTT법평고인지방간충질간세포재지가재료상적점부화증식정황，지가재료대세포적독성작용；광경화전경분별관찰인지방간충질간세포재지가재료상적점부화생장과정，이급세포형태학변화。 　　결과여결론：인지방간충질간세포접충우지가재료후，능구신속재재료상점부、증식，기점부솔평균위86.5%；광경화소묘전경현시인지방간충질간세포재지가재료상점부성량호，수착시간적추이，세포축점증다，가이천이진입지가내부병균균분포。결과표명경니평대세포적독성저，교련후적지가재료여인지방간충질간세포구유량호적체외생물상용성。
BACKGROUND:Low toxicity of Genipin has certain species and cellspecificity. Biocompatibility of Genipin cross-linked type I colagen with human adipose-derived stem cels is essential for construction of tissue-engineered adipose. OBJECTIVE:To investigate the bbiocompatibility of Genipin cross-linked type I colagen with human adipose-derived stem cels. METHODS:Human adipose-derived stem cels were isolated and cultured to the third generation, and the cels were seeded on Genipin cross-linked type I colagen scaffold. MTT assay was used to evaluate the adhesion and proliferation of cels on the scaffold, and the toxic effects of Genipin cross-linked type I colagen on human adipose-derived stem cels. Optical microscopy and scanning electron microscopy were utilized to observe the adhesion and growth process of human adipose-derived stem cels on the scaffold as wel as the morphological changes of cels. RESULTS AND CONCLUSION:Human adipose-derived stem cels could adhere to the scaffold immediately after seeded and increase gradualy on the scaffold, with the average adhesion rate of 86.5%. Optical microscopy and scanning electron microscopy showed that human adipose-derived stem cels adhered wel on the scaffold. The cels increased gradualy over time, and could migrate into the scaffold, and distribute evenly with the passage of time when observed with optical microscopy. The result showed Genipin possesses very low cytotoxicity to the cels, and the outstanding biocompatibility is found between the cels and scaffoldin vitro after cross-linked with Genipin.