中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
34期
5413-5417
,共5页
耿海霞%郭秀娟%钱君荣%封伟
耿海霞%郭秀娟%錢君榮%封偉
경해하%곽수연%전군영%봉위
组织工程%成骨细胞%动物模型%支架基金资助
組織工程%成骨細胞%動物模型%支架基金資助
조직공정%성골세포%동물모형%지가기금자조
tissue engineering%osteoblasts%models,animal%stents
背景：羟基磷灰石/凝胶纳米新型复合物具备与天然骨相同的机械强度，但对骨缺损的修复能力与成骨效果还有待于进一步研究证实。<br> 目的：探索羟基磷灰石/凝胶纳米复合物在兔颅骨缺损中的修复效果。<br> 方法：建立兔颅顶骨洞型骨缺损模型，缺损区植入羟基磷灰石/凝胶新型复合物，空白缺损设为阴性对照，自体颅骨设为阳性对照。于植入后4，8，12周通过X射线片与CT观察分析骨缺损修复状况。<br> 结果与结论：X 射线片观察显示，植入后8周，大鼠自体颅骨修复区与周围正常的骨组织形态类似；羟基磷灰石/凝胶纳米复合物修复区中央位置骨质致密，形态接近正常骨组织，边缘略显模糊，植入后12周，羟基磷灰石/凝胶纳米复合物与骨缺损边缘更加模糊，复合物中心呈不连贯状态，空白缺损组可观察到明显的规则的透射阴影。植入后12周 CT 检测显示，羟基磷灰石/凝胶新型复合物缺损边缘部分紧密融合，与周围的正常骨组织结合为一体。结果说明，羟基磷灰石/凝胶新型仿生复合物在兔颅骨缺损中可取得较好的修复效果。
揹景：羥基燐灰石/凝膠納米新型複閤物具備與天然骨相同的機械彊度，但對骨缺損的脩複能力與成骨效果還有待于進一步研究證實。<br> 目的：探索羥基燐灰石/凝膠納米複閤物在兔顱骨缺損中的脩複效果。<br> 方法：建立兔顱頂骨洞型骨缺損模型，缺損區植入羥基燐灰石/凝膠新型複閤物，空白缺損設為陰性對照，自體顱骨設為暘性對照。于植入後4，8，12週通過X射線片與CT觀察分析骨缺損脩複狀況。<br> 結果與結論：X 射線片觀察顯示，植入後8週，大鼠自體顱骨脩複區與週圍正常的骨組織形態類似；羥基燐灰石/凝膠納米複閤物脩複區中央位置骨質緻密，形態接近正常骨組織，邊緣略顯模糊，植入後12週，羥基燐灰石/凝膠納米複閤物與骨缺損邊緣更加模糊，複閤物中心呈不連貫狀態，空白缺損組可觀察到明顯的規則的透射陰影。植入後12週 CT 檢測顯示，羥基燐灰石/凝膠新型複閤物缺損邊緣部分緊密融閤，與週圍的正常骨組織結閤為一體。結果說明，羥基燐灰石/凝膠新型倣生複閤物在兔顱骨缺損中可取得較好的脩複效果。
배경：간기린회석/응효납미신형복합물구비여천연골상동적궤계강도，단대골결손적수복능력여성골효과환유대우진일보연구증실。<br> 목적：탐색간기린회석/응효납미복합물재토로골결손중적수복효과。<br> 방법：건립토로정골동형골결손모형，결손구식입간기린회석/응효신형복합물，공백결손설위음성대조，자체로골설위양성대조。우식입후4，8，12주통과X사선편여CT관찰분석골결손수복상황。<br> 결과여결론：X 사선편관찰현시，식입후8주，대서자체로골수복구여주위정상적골조직형태유사；간기린회석/응효납미복합물수복구중앙위치골질치밀，형태접근정상골조직，변연략현모호，식입후12주，간기린회석/응효납미복합물여골결손변연경가모호，복합물중심정불련관상태，공백결손조가관찰도명현적규칙적투사음영。식입후12주 CT 검측현시，간기린회석/응효신형복합물결손변연부분긴밀융합，여주위적정상골조직결합위일체。결과설명，간기린회석/응효신형방생복합물재토로골결손중가취득교호적수복효과。
BACKGROUND:Hydroxyapatite/gel nano-composite has the same mechanical strength to the natural bone, but its ability to repair bone defects and osteogenic effect need to be confirmed by further studies. <br> OBJECTIVE:To explore the repair effect of hydroxyapatite/gel bionic composite in skul defects of rabbits. <br> METHODS: The hole-like calvarium defect models were established in rabbits, and treated with hydroxyapatite/ gel composites (hydroxyapatite/gel group), autologous skul as positive control (autologous bone group) and <br> nothing as negative control (blank group). The repairing condition in the skul defect areas were observed and analyzed by X-ray and CT at 4, 8, 12 weeks after implantation. <br> RESULTS AND CONCLUSION: After 8 weeks, X-ray assessment showed that normal-like bone tissue appeared in the defect region of the autologous bone group; in the hydroxyapatite/gel group, dense bone with similar <br> morphology to normal bone tissue was found in the central site of defect region, and the boundary was slightly blurred. After 12 weeks, the hydroxyapatite/gel showed blurred edge compared with autologous bone, and the center of the composite was disconnected; in the blank group, a clear and regular transmitted shadow was <br> observed. After 12 weeks, CT examination showed that the hydroxyapatite/gel was connected tightly with the surrounding normal bone tissue. As a new bionic composite, the hydroxyapatite/gel can achieve good effect in repairing skul defects of rabbits.
