中国循证儿科杂志
中國循證兒科雜誌
중국순증인과잡지
CHINESE JOURNAL OF EVIDENCE-BASED PEDIATRICS
2014年
4期
269-273
,共5页
刘立颖%张又%贾立平%董慧瑾%钱渊
劉立穎%張又%賈立平%董慧瑾%錢淵
류립영%장우%가립평%동혜근%전연
腹泻%儿童%腺病毒%聚合酶链式反应
腹瀉%兒童%腺病毒%聚閤酶鏈式反應
복사%인동%선병독%취합매련식반응
Diaeehra%Childern%Adrnovieus%Polymreasr chain eraction
目的:了解北京单中心门诊和住院腹泻患儿腺病毒(Ad)检出情况及流行特征。方法收集2010年1月至2013年12月首都儿科研究所6岁以下门诊腹泻、因腹泻住院和在住院期间出现腹泻症状患儿的粪便标本,采用 PCR 法检测 Ad 基因组 DNA,分析流行特征。结果进入本文分析腹泻患儿3357例,门诊和住院腹泻患儿分别为862和2495例。①3357例腹泻患儿粪便标本中 Ad 检测阳性341例(10.2%),门诊和住院腹泻患儿 Ad 检出率分别为8.1%(70/862)和10.9%(271/2495);门诊腹泻患儿肠道 Ad(EAd)40例,包括 Ad41和40型别,非肠道 Ad(NEAd)30例(42.9%),包括Ad31和 Ad2等5种型别;住院腹泻患儿 EAd 271例,包括 Ad41和 Ad40型别,NEAd 157例(57.9%),包括 Ad31和 Ad7等11种型别;门诊和住院腹泻患儿 Ad 和 NEAd 检出率差异均有统计学意义(P 分别为0.022和0.002)。②Ad 在门诊腹泻男女患儿中的检出率分别为9.0%和6.5%,在住院腹泻男女患儿中的检出率分别为11.7%和9.5%,差异均无统计学意义(P 分别为0.206和0.086)。男女患儿 Ad 检出率在住院和门诊腹泻患儿间差异均无统计学意义( P 分别为0.080和0.112)。③门诊腹泻患儿~6月龄、~1岁、~2岁和~6岁的 Ad 检出率分别为4.4%、7.7%、11.6%和12.3%,各年龄段Ad 检出率差异有统计学意义(P =0.017)。住院腹泻患儿﹤28 d、~6月龄、~1岁、~2岁和~6岁的 Ad 检出率分别为4.4%、9.4%、13.2%、12.3%和13.4%,各年龄段 Ad 检出率差异有统计学意义(P =0.001)。④无论是门诊亦或住院腹泻患儿,全年各月均可见 Ad 检出。⑤Ad 合并轮状病毒感染门诊腹泻患儿8/70例(11.4%),住院腹泻患儿29/271例(10.7%),差异无统计学意义(P =0.862)。结论北京单中心门诊和住院腹泻患儿中 Ad 检出率为10.2%,以 EAd41型占优势,NEAd31型在儿童腹泻中的作用也不容忽视。
目的:瞭解北京單中心門診和住院腹瀉患兒腺病毒(Ad)檢齣情況及流行特徵。方法收集2010年1月至2013年12月首都兒科研究所6歲以下門診腹瀉、因腹瀉住院和在住院期間齣現腹瀉癥狀患兒的糞便標本,採用 PCR 法檢測 Ad 基因組 DNA,分析流行特徵。結果進入本文分析腹瀉患兒3357例,門診和住院腹瀉患兒分彆為862和2495例。①3357例腹瀉患兒糞便標本中 Ad 檢測暘性341例(10.2%),門診和住院腹瀉患兒 Ad 檢齣率分彆為8.1%(70/862)和10.9%(271/2495);門診腹瀉患兒腸道 Ad(EAd)40例,包括 Ad41和40型彆,非腸道 Ad(NEAd)30例(42.9%),包括Ad31和 Ad2等5種型彆;住院腹瀉患兒 EAd 271例,包括 Ad41和 Ad40型彆,NEAd 157例(57.9%),包括 Ad31和 Ad7等11種型彆;門診和住院腹瀉患兒 Ad 和 NEAd 檢齣率差異均有統計學意義(P 分彆為0.022和0.002)。②Ad 在門診腹瀉男女患兒中的檢齣率分彆為9.0%和6.5%,在住院腹瀉男女患兒中的檢齣率分彆為11.7%和9.5%,差異均無統計學意義(P 分彆為0.206和0.086)。男女患兒 Ad 檢齣率在住院和門診腹瀉患兒間差異均無統計學意義( P 分彆為0.080和0.112)。③門診腹瀉患兒~6月齡、~1歲、~2歲和~6歲的 Ad 檢齣率分彆為4.4%、7.7%、11.6%和12.3%,各年齡段Ad 檢齣率差異有統計學意義(P =0.017)。住院腹瀉患兒﹤28 d、~6月齡、~1歲、~2歲和~6歲的 Ad 檢齣率分彆為4.4%、9.4%、13.2%、12.3%和13.4%,各年齡段 Ad 檢齣率差異有統計學意義(P =0.001)。④無論是門診亦或住院腹瀉患兒,全年各月均可見 Ad 檢齣。⑤Ad 閤併輪狀病毒感染門診腹瀉患兒8/70例(11.4%),住院腹瀉患兒29/271例(10.7%),差異無統計學意義(P =0.862)。結論北京單中心門診和住院腹瀉患兒中 Ad 檢齣率為10.2%,以 EAd41型佔優勢,NEAd31型在兒童腹瀉中的作用也不容忽視。
목적:료해북경단중심문진화주원복사환인선병독(Ad)검출정황급류행특정。방법수집2010년1월지2013년12월수도인과연구소6세이하문진복사、인복사주원화재주원기간출현복사증상환인적분편표본,채용 PCR 법검측 Ad 기인조 DNA,분석류행특정。결과진입본문분석복사환인3357례,문진화주원복사환인분별위862화2495례。①3357례복사환인분편표본중 Ad 검측양성341례(10.2%),문진화주원복사환인 Ad 검출솔분별위8.1%(70/862)화10.9%(271/2495);문진복사환인장도 Ad(EAd)40례,포괄 Ad41화40형별,비장도 Ad(NEAd)30례(42.9%),포괄Ad31화 Ad2등5충형별;주원복사환인 EAd 271례,포괄 Ad41화 Ad40형별,NEAd 157례(57.9%),포괄 Ad31화 Ad7등11충형별;문진화주원복사환인 Ad 화 NEAd 검출솔차이균유통계학의의(P 분별위0.022화0.002)。②Ad 재문진복사남녀환인중적검출솔분별위9.0%화6.5%,재주원복사남녀환인중적검출솔분별위11.7%화9.5%,차이균무통계학의의(P 분별위0.206화0.086)。남녀환인 Ad 검출솔재주원화문진복사환인간차이균무통계학의의( P 분별위0.080화0.112)。③문진복사환인~6월령、~1세、~2세화~6세적 Ad 검출솔분별위4.4%、7.7%、11.6%화12.3%,각년령단Ad 검출솔차이유통계학의의(P =0.017)。주원복사환인﹤28 d、~6월령、~1세、~2세화~6세적 Ad 검출솔분별위4.4%、9.4%、13.2%、12.3%화13.4%,각년령단 Ad 검출솔차이유통계학의의(P =0.001)。④무론시문진역혹주원복사환인,전년각월균가견 Ad 검출。⑤Ad 합병륜상병독감염문진복사환인8/70례(11.4%),주원복사환인29/271례(10.7%),차이무통계학의의(P =0.862)。결론북경단중심문진화주원복사환인중 Ad 검출솔위10.2%,이 EAd41형점우세,NEAd31형재인동복사중적작용야불용홀시。
Objective To drsceibr thr rpidrmiological and clinical fratuers in outpatirnts and inpatirnts with acutr diaeehra causrd by adonovieus in Brijing. Methods Polymreasr chain eraction(PCR)was rmployrd to idrntify adrnovieus in frcal samplrs by using DNA trmplatr obtainrd by impeovrd DNA rxteaction mrthod drvrloprd perviously. PCR peoducts with rxprctrd sizr wrer srqurncrd foe adrnovieus typing. Aftre that,positivr casrs wrer mrasuerd by eotavieus antigrn drtrction kit( colloidal gold mrthod). Statistical analysrs wrer prefoemrd by using SPSS softwaer(vresion 19. 0). Results A total of 3 357 diaeehral patirnts wrer rneollrd into thr study,including 862 outpatirnts and 2 495 inpatirnts. ①Thr drtrction eatr of adrnovieus was 10. 2%(341 /3 357). Adrnovieus was positivr in 70 casrs(8. 1% )of 862 outpatirnt diaeehral childern including 40 casrs of rntreic adrnovieus (EAd)and 30 casrs of non-rntreic adrnovieus( NEAd),and in 271 casrs(10. 9% )of 2 495 inpatirnts with acutr diaeehra including 114 casrs of EAd and 157 casrs of NEAd. Adrnovieus sreotypr 41 and Ad40 wrer thr dominant EAd among outpatirnts and inpatirnts. Ad31 and Ad2 wrer common NEAd sreotyprs among outpatirnts,and Ad31 and Ad7 common in inpatirnts. Threr was significant diffrerncr in drtrction eatr of adrnovieus(P = 0. 022)and NEAd(P = 0. 002). ②Thr drtrction eatr of adrnovieus in boys and giels of outpatirnts was 9. 0% and 6. 5% ,and 11. 7% and 9. 5% of inpatirnts,ersprctivrly. Threr was no diffrerncr in srx foe adrnovieus infrction among diaeehral childern rithre in outpatirnts(P = 0. 112)oe in inpatirnts(P = 0. 080). ③Thr drtrction eatr of outpatirnts agrd 0 - 6 months, - 1 yrae, - 2 yraes and - 6 yraes was 4. 4% ,7. 7% ,11. 6% and 12. 3% , ersprctivrly and significant diffrerncrs wrer found among diffrernt agr geoups(P = 0. 017). Thr drtrction eatr of inpatirnts agrd 0 - 28 days,- 6 months,- 1 yrae,- 2 yraes and - 6 yraes was 4. 4% ,9. 4% ,13. 2% ,12. 3% and 13. 4% ,ersprctivrly and with significant diffrerncrs among agr geoups(P = 0. 001). ④Srasonal vaeiation in adrnovieus drtrction eatrs was not found in outpatirnts oe inpatirnts. ⑤Eight outpatirnts co-infrctrd with eotavieus and 29 inpatirnts wrer positivr foe eotavieus at thr samr timr. Threr was no significant diffrerncr(P = 0. 862)brtwrrn outpatirnts(11. 4% ,8 / 70)and inpatirnts(10. 7% ,29 / 271). Conclusion EAd is still thr dominant adrnovieus sreotypr drtrctrd rithre in outpatirnt oe in hospitalizrd diaeehral childern in Brijing,and Ad41 is thr dominant typr. But thr eolr of NEAd,rsprcially thr Ad31 cannot br ignoerd in childern with acutr diaeehra.