CAJ | 학술논문

目的：应用随机引物扩增多态性DNA技术（ random amplified polymorphic DNA ， RAPD）对大耳白黑眼兔（ white hair black eyes rabbit ， WHBE rabbit ）、日本大耳白兔（ Japanese white rabbit ， JW rabbit ）和新西兰兔（New Zealand white rabbit， NZW rabbit）3个实验兔品系进行遗传分析。方法选用90只实验兔的皮肤组织样品提取基因组DNA，用60个随机引物对实验兔基因组DNA进行PCR扩增，根据电泳结果筛选出多态性较高的引物进行RAPD-PCR分析，再利用Popgene 3．2统计软件对3个品系的扩增条带进行遗传分析，获得实验数据。结果分析结果表明：（1）60个随机引物中筛选出25个多态性较高的引物，3个品系实验兔共检测到493个扩增片段，长度在100～1800 bp之间，筛选的25个引物中，其中16个引物既可扩增出3个品系共同的DNA条带，也可扩增出WHBE兔特有的特征条带；（2） WHBE兔位点数为234个，其中多态位点数166个，多态位点比为70．94％，JW兔位点数为228个，其中多态位点数122个，多态位点比为53．51％，NZW兔位点数为231个，其中多态位点数94个，多态位点比为40．69％；（3）三个群体的Shannon多样性指数分别为0．3385，0．2222和0．1905；（4） JW兔和NZW兔的遗传相似系数最高，为0．8443，其次为WHBE兔和JW兔的遗传相似系数，为0．8204，WHBE兔和NZW兔的遗传相似系数最低，为0．7862。结论结果表明WHBE兔与JW兔和NZW兔之间有遗传的相似性，也存在着遗传差异，应用RAPD技术可以很好地检测实验兔不同品系之间以及同一品系不同个体之间的亲缘关系。
목적：응용수궤인물확증다태성DNA기술（ random amplified polymorphic DNA ， RAPD）대대이백흑안토（ white hair black eyes rabbit ， WHBE rabbit ）、일본대이백토（ Japanese white rabbit ， JW rabbit ）화신서란토（New Zealand white rabbit， NZW rabbit）3개실험토품계진행유전분석。방법선용90지실험토적피부조직양품제취기인조DNA，용60개수궤인물대실험토기인조DNA진행PCR확증，근거전영결과사선출다태성교고적인물진행RAPD-PCR분석，재이용Popgene 3．2통계연건대3개품계적확증조대진행유전분석，획득실험수거。결과분석결과표명：（1）60개수궤인물중사선출25개다태성교고적인물，3개품계실험토공검측도493개확증편단，장도재100～1800 bp지간，사선적25개인물중，기중16개인물기가확증출3개품계공동적DNA조대，야가확증출WHBE토특유적특정조대；（2） WHBE토위점수위234개，기중다태위점수166개，다태위점비위70．94％，JW토위점수위228개，기중다태위점수122개，다태위점비위53．51％，NZW토위점수위231개，기중다태위점수94개，다태위점비위40．69％；（3）삼개군체적Shannon다양성지수분별위0．3385，0．2222화0．1905；（4） JW토화NZW토적유전상사계수최고，위0．8443，기차위WHBE토화JW토적유전상사계수，위0．8204，WHBE토화NZW토적유전상사계수최저，위0．7862。결론결과표명WHBE토여JW토화NZW토지간유유전적상사성，야존재착유전차이，응용RAPD기술가이흔호지검측실험토불동품계지간이급동일품계불동개체지간적친연관계。
Objective To analyze the genetic variation among white hair black eyes (WHBE) rabbit, Japanese white ( JW) rabbit and New Zealand white ( NZW) rabbit using random amplified polymorphic DNA ( RAPD) technique . Methods Thirty rabbits (male/female 1∶1) of each strain were used in this study.The genomic DNA was extracted from 90 rabbits.Sixty arbitrary primers were used to amplify DNA of rabbits with RAPD-PCR method.Based on the preliminary experiments , polymorphic primers were selected to analyze the genetic variation among the three rabbit strains .The experi-mental data were analyzed using Popgene 3.2 software.Results (1) Twenty-five polymorphic primers were selected among 60 arbitrary primers.493 amplified fragments were detected ranging from 100 bp to 1800 bp.Sixteen primers among 25 arbitrary primers could not only amplify the common DNA bands of 3 rabbit breeds , but also amplify particular alleles in the WHBE rabbit.(2) 234 RAPD sites were detected by agarose gel electrophoresis in WHBE rabbit , among which 166 sites were polymorphic , accounting for 70.94%.228 RAPD sites were detected by agarose gel electrophoresis in the JW rabbit, while 122 sites of them were polymorphic , accounting for 53.51%.231 RAPD sites were detected by agarose gel e-lectrophoresis in the NZW rabbits , with 94 sites being polymorphic, accounting for 40.69%.(3) The Shannon genetic di-versity index of WHBE rabbit, JW rabbit and NZW rabbit was 0.3385, 0.2222 and 0.1905, respectively.(4) The genet-ic similarity between JW rabbit and NZW rabbit was highest among the three rabbit breeds (0.8443), followed by that be-tween WHBE rabbit and JW rabbit (0.8204), and the genetic similarity between WHBE rabbit and NZW rabbit (0.7862) was the lowest .Conclusions Our results demonstrate that there are both genetic similarities and genetic variations among WHBE rabbit, JW rabbit and NZW rabbit .The RAPD technique can be used to delect the genetic relationships among dif-ferent breeds and different individuals of the same breed of rabbits .