CAJ | 학술논문

目的：通过注射低剂量四氯化碳（ carbon tetrachloride ，CCl4）建立B/C小鼠肝损伤模型。方法正常B/C小鼠随机分为正常对照组、油对照组、CCl4模型组。正常对照组常规饲养；油对照组腹腔注射鲁花花生油（10μL/g，1次/3天，连续6周）；CCl4模型组腹腔注射0.5％CCl4（10μL/g，1次/3天，连续6周）。第6周，各组小鼠检测血清AST、ALT浓度，HE及Masson染色后观察小鼠肝脏结构、细胞形态及纤维化程度。结果第6周CCl4模型组小鼠血清ALT（P＝0.00）、AST（P＝0.00）浓度极显著性增高，HE及Masson染色显示CCl4模型组小鼠肝上皮细胞呈广泛性空泡样变及大量坏死，肝小叶内出现明显的条索样纤维增生，其纤维化程度评分显著性升高（P ＝0.00），纤维显色积分光密度值极显著性增高（P ＝0.00）。结论注射低剂量CCl4可以诱导B/C小鼠肝损伤模型，实验模型具备肝损伤和肝纤维化病理特征。
목적：통과주사저제량사록화탄（ carbon tetrachloride ，CCl4）건립B/C소서간손상모형。방법정상B/C소서수궤분위정상대조조、유대조조、CCl4모형조。정상대조조상규사양；유대조조복강주사로화화생유（10μL/g，1차/3천，련속6주）；CCl4모형조복강주사0.5％CCl4（10μL/g，1차/3천，련속6주）。제6주，각조소서검측혈청AST、ALT농도，HE급Masson염색후관찰소서간장결구、세포형태급섬유화정도。결과제6주CCl4모형조소서혈청ALT（P＝0.00）、AST（P＝0.00）농도겁현저성증고，HE급Masson염색현시CCl4모형조소서간상피세포정엄범성공포양변급대량배사，간소협내출현명현적조색양섬유증생，기섬유화정도평분현저성승고（P ＝0.00），섬유현색적분광밀도치겁현저성증고（P ＝0.00）。결론주사저제량CCl4가이유도B/C소서간손상모형，실험모형구비간손상화간섬유화병리특정。
Objective The aim of this study was to establish a mouse model of chronic hepatic injury induced by low dose carbon tetrachloride ( CCl4 ) .Methods Twenty SPF male B/C mice ( body weight 18-20 g) were randomly di-vided into three groups including the CCl 4-treated group , oil-treated group and non-treated control group ( n=5/group ) . Mice in the CCl4-treated group were intraperitoneally injected with 0.5% CCl4 prepared in oil.Mice in the oil group re-ceived intraperitoneal injection of oil .Mice in the non-treated control group were left untreated .After 6 weeks, the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured, as well structure, cellular morphology and degree of fibrosis of the hepatic tissues were examined by histology with HE and Masson staining .Results After low dose CCL4 treatment, the serum ALT and AST were significantly increased (P =0.00).Histology with HE stai-ning showed extensive vacuolar degeneration of hepatic epithelial cells and large number of necrotic foci .Histology with Masson staining revealed fibrous hyperplasia mainly located around hepatic lobules .Quantitative analysis of the fibrosis showed that the degree of fibrosis and the integrated optical density of fibrosis were significantly increased after CCl 4 induc-tion( P=0.00) .Conclusion Low dose carbon tetrachloride can induce hepatic injury in B /C mouse models presenting pathological changes of hepatic injury and fibrosis .