CAJ | 학술논문

目的：探讨电针促进局灶脑缺血/再灌注后缺血海马区血管再生的机制。方法180只雄性SD大鼠随机分为假手术组、模型组、电针组、CXCR4特异性拮抗剂AMD3100药物组、AMD3100＋电针组。线栓法制备右侧局灶脑缺血/再灌注模型。取大鼠“百会”穴（ GV 20）及左侧“四关”穴（合谷LI 4/太冲LR 3）为电针穴位，刺激时间为30 min/d。采用逆转录聚合酶链反应法（ RT-PCR）检测各组缺血海马区SDF-1α、CXCR4 mRNA表达，免疫荧光双标法检测CD34＋VEGFR2＋EPCs源性血管的表达。结果与假手术组比较，模型组与电针组SDF-1α、CX-CR4 mRNA表达明显增高（P＜0.05），其中电针组各时间点相对模型组增高更为显著（P＜0.05）。 AMD3100＋电针组缺血海马SDF-1α、CXCR4 mRNA表达在再灌注后1 d时明显高于电针组（ P＜0.05），但后逐渐下降，7 d时明显低于电针组（ P＜0.01）。与模型组比较，电针组再灌注3 d、7 d海马CD34＋VEGFR2＋EPCs源性血管表达明显增多（ P＜0.05）。与电针组比较，AMD3100＋电针组再灌注后7 d CD34＋VEGFR2＋EPCs源性血管表达明显下降（ P＜0.01）。 CD34＋VEGFR2＋血管表达变化与SDF-1α的表达变化显著相关（R＝0.784，P＜0.01）。结论电针可通过上调局灶脑缺血/再灌注大鼠缺血海马区SDF-1α/CXCR4的表达，促进血管再生。
목적：탐토전침촉진국조뇌결혈/재관주후결혈해마구혈관재생적궤제。방법180지웅성SD대서수궤분위가수술조、모형조、전침조、CXCR4특이성길항제AMD3100약물조、AMD3100＋전침조。선전법제비우측국조뇌결혈/재관주모형。취대서“백회”혈（ GV 20）급좌측“사관”혈（합곡LI 4/태충LR 3）위전침혈위，자격시간위30 min/d。채용역전록취합매련반응법（ RT-PCR）검측각조결혈해마구SDF-1α、CXCR4 mRNA표체，면역형광쌍표법검측CD34＋VEGFR2＋EPCs원성혈관적표체。결과여가수술조비교，모형조여전침조SDF-1α、CX-CR4 mRNA표체명현증고（P＜0.05），기중전침조각시간점상대모형조증고경위현저（P＜0.05）。 AMD3100＋전침조결혈해마SDF-1α、CXCR4 mRNA표체재재관주후1 d시명현고우전침조（ P＜0.05），단후축점하강，7 d시명현저우전침조（ P＜0.01）。여모형조비교，전침조재관주3 d、7 d해마CD34＋VEGFR2＋EPCs원성혈관표체명현증다（ P＜0.05）。여전침조비교，AMD3100＋전침조재관주후7 d CD34＋VEGFR2＋EPCs원성혈관표체명현하강（ P＜0.01）。 CD34＋VEGFR2＋혈관표체변화여SDF-1α적표체변화현저상관（R＝0.784，P＜0.01）。결론전침가통과상조국조뇌결혈/재관주대서결혈해마구SDF-1α/CXCR4적표체，촉진혈관재생。
Objective To explore the effect of electroacupuncture on CD 34 +VEGFR2 +endothelial progenitor cell (EPC)-derived vessels and stromal cell-derived factor-1α(SDF-1α)/CXCR4, and study its mechanism of promoting an-giogenesis in hippocampus after focal cerebral ischemia /reperfusion .Methods A total of 180 healthy male adult Sprague Dawley (SD) rats were randomly divided into sham operation (sham) group, model (I/R) group, electroacupuncture (I/RE) group, I/RE plus AMD3100 (A specific antagonist of CXCR4) group (I/REA) and AMD3100 (I/RA) group. The rats received filament occlusion of the right middle cerebral artery for 2 hours followed by reperfusion .Electroacupunc-ture was applied to “Baihui” (GV20)/“Siguan” (Hegu LI 4/Taichong LR 3) acupoints for 30 min, once a day.The mR-NA expression of SDF-1αand CXCR4 were detected by reverse transcription-polymerase chain reaction ( RT-PCR) .Double immunofluorescence was used to stain CD 34 +VEGFR2 +EPC-derived vessels.Results Compared with the sham group, the mRNA expressions of SDF-1αand CXCR4 were significantly upregulated in I/R and I/RE group ( P<0.05 ) , but that in I/RE group was more significantly increased than I/R group(P<0.05).In addition, the mRNA expression of SDF-1αand CXCR4 were highly increased on day 1 in the I/REA group than that of I/RE group, but decreased than that of I/RE group on day 7 after reperfusion (P<0.01).CD34 +VEGFR2 +EPCs-derived vessels were obviously increased on 3d and 7d in the I/RE group compared with that of the I/R group, and significantly decreased on 7d in the I/REA group compared with that of the I/RE group ( P<0.01) .Conclusions Electroacupuncture can effectively promote an-giogenesis through upregulating the expression of SDF-1αand CXCR4 in rat ischemic hippocampus after focal cerebral is-chemia/reperfusion.