CAJ | 학술논문

目的：对从新疆实验动物研究中心饲养的封闭群灰仓鼠体内分离到的1株鞭毛虫进行形态学鉴定及基因鉴定。方法取灰仓鼠回盲部内容物进行直接涂片和常规姬姆萨染色后镜检观察，提取虫体总DNA，PCR扩增该鞭毛虫的16S rRNA基因，测序后与国外已报道的鞭毛虫进行核酸同源性分析，并应用MEGA5.22软件绘制系统发育进化树。结果形态学观察表明分离到的鞭毛虫为鼠三毛滴虫。测序后核酸同源性分析表明鼠三毛滴虫新疆灰仓鼠分离株16S rRNA序列与国外已报道的三毛滴虫高度同源。系统发育进化树表明鼠三毛滴虫新疆灰仓鼠分离株序列与已报道的鼠三毛滴虫16S rRNA（序列号AY886846.1）位于同一进化分支，与其他相关三毛滴虫亲缘关系较远。结论形态学鉴定和16 S rRNA基因分析表明，此次从新疆实验动物研究中心饲养的封闭群灰仓鼠体内分离到的鞭毛虫为鼠三毛滴虫。
목적：대종신강실험동물연구중심사양적봉폐군회창서체내분리도적1주편모충진행형태학감정급기인감정。방법취회창서회맹부내용물진행직접도편화상규희모살염색후경검관찰，제취충체총DNA，PCR확증해편모충적16S rRNA기인，측서후여국외이보도적편모충진행핵산동원성분석，병응용MEGA5.22연건회제계통발육진화수。결과형태학관찰표명분리도적편모충위서삼모적충。측서후핵산동원성분석표명서삼모적충신강회창서분리주16S rRNA서렬여국외이보도적삼모적충고도동원。계통발육진화수표명서삼모적충신강회창서분리주서렬여이보도적서삼모적충16S rRNA（서렬호AY886846.1）위우동일진화분지，여기타상관삼모적충친연관계교원。결론형태학감정화16 S rRNA기인분석표명，차차종신강실험동물연구중심사양적봉폐군회창서체내분리도적편모충위서삼모적충。
Objective To conduct morphological observation and gene identification of the strain of flagellate iso -lated from Cricetulus migratorius in the Xinjiang Research Center for Experimental Animals .Methods The ileocecal con-tents of C.migratorius were microscopically examined on direct smear with Wright-Giemsa staining , and the total RNA iso-lated from Xinjiang C.migratorius was extracted and 16S rRNA was amplified by PCR , and then sequenced .Furthermore the homology was compared and the phylogenetic tree was developed using MEGA 5.22 software.Results Morphological observation indicated that the isolated flagellate was Tritrichomonas muris.The 16S rRNA gene sequence of the Xinjiang C. migratorius isolate shared highly homology with that of other Tritrichomonas.Phylogenetic tree analysis indicated that the 16S rRNA gene of Xinjiang C.migratorius isolate was classified into a subgroup with T.muris 16S rRNA (U85966.1), but was relatively distant relative from other related tritrichomonas.Conclusions The flagellate isolated from Xinjiang C. migratorius is identified to be T.muris by both morphological observation and 16S rRNA gene analysis.