CAJ | 학술논문

目的：采用1 H核磁共振谱分析结合偏最小二乘分析（ PLS-DA）方法研究自发性高血压大鼠和Wist-ar大鼠尿液的成分谱差异，寻找可能的生物标记物。方法选取自发性高血压大鼠和Wistar大鼠，连续4周收集尿液，以1 H-NMR分析两组大鼠的尿液成分，使用PLS-DA方法进行模式识别，通过OPLS-DA的相关系数寻找差异标记物。结果 PLS-DA方法处理高血压大鼠和Wistar大鼠的1 H-NMR谱数据显示，两组1 HNMR谱数据可以在得分图中明显区分，且代谢趋势在连续4周测定均非常稳定，说明自发性高血压大鼠有着特定的代谢模式，通过OPLS相关系数分析出体内部分氨基酸代谢产物和葡萄糖等能量代谢物质明显异于正常大鼠。结论1 H-NMR结合PLS-DA模式识别的代谢组学方法具有研究复杂条件下机体病理生理变化的优势，为了解高血压大鼠的代谢特征提供了科学依据。
목적：채용1 H핵자공진보분석결합편최소이승분석（ PLS-DA）방법연구자발성고혈압대서화Wist-ar대서뇨액적성분보차이，심조가능적생물표기물。방법선취자발성고혈압대서화Wistar대서，련속4주수집뇨액，이1 H-NMR분석량조대서적뇨액성분，사용PLS-DA방법진행모식식별，통과OPLS-DA적상관계수심조차이표기물。결과 PLS-DA방법처리고혈압대서화Wistar대서적1 H-NMR보수거현시，량조1 HNMR보수거가이재득분도중명현구분，차대사추세재련속4주측정균비상은정，설명자발성고혈압대서유착특정적대사모식，통과OPLS상관계수분석출체내부분안기산대사산물화포도당등능량대사물질명현이우정상대서。결론1 H-NMR결합PLS-DA모식식별적대사조학방법구유연구복잡조건하궤체병리생리변화적우세，위료해고혈압대서적대사특정제공료과학의거。
Objective To investigate the application of metabolomics in research of the metabolic profiling of spontaneously hypertensive rats ( SHR) and Wistar rats using NMR combined with PLS-DA.Methods SHRs were fed with normal diet for 1 week and then the urines of normal rats and SHR hypertensive model rats were collected at 1, 2, 3, and 4 weeks.The metabolic profiles were analyzed using 1H-NMR.Partial least squares discriminant analysis (PLS-DA) was used to determine the differential biomarkers .Results The systolic blood pressure (SBP) of SHRs were significantly higher than that of normal rats.Using PLS-DA and orthogonal partial least squares discriminant analysis (OPLS-DA), it was also possible to distinguish them in the principal components score plot .In the SHRs, the levels of some amino acids and some small molecular substances were higher than those in the control rats .Conclusions NMR combined with PLS-DA is a promising approach to provide the information on metabolic changes related to the pathophysiological processes in spontaneously hypertensive rats .