CAJ | 학술논문

目的：用体重检测、空腹血糖检测、宏观表征、旷场实验行为学评价糖尿病兼抑郁症的大鼠模型。方法采用高脂饲料喂养加腹腔注射小剂量链脲佐菌素（ STZ）的方法制备2型糖尿病模型，在其基础上再用21 d慢性束缚的方法建立糖尿病兼抑郁症大鼠模型。将32只Wistar大鼠随机分为3组（ n ＝8）：正常组（N组），2型糖尿病组（T组），2型糖尿病兼抑郁症组（T＋D组）。2型糖尿病模型建立后，在慢性束缚的第0、7、14、21天检测大鼠的空腹血糖和体重，并对大鼠的宏观表征、饮食量、粪便、小便、精神状态进行观察，在第21天利用行为学设备分析软件，对大鼠旷场实验进行分析，检测大鼠的抑郁程度，验证评价2型糖尿病兼抑郁症大鼠模型是否成功。结果给予高脂饲料及腹腔注射STZ制备2型糖尿病模型后，T＋D组大鼠的毛发散乱，无光泽，活动迟缓，进食量、饮水量增加，粪便尿量增加，精神萎靡。第0、7、14、21天T组和T＋D组组大鼠体重均下降，与N组比较差异有显著性（P＜0.05；P＜0.01），21d慢性束缚刺激后，T＋D组体重比T组大鼠体重增加较慢，差异有显著性（P＜0.05）；第0、7、14、21天，T组和T＋D组大鼠血糖均升高，与N组比较差异有显著性（ P＜0.01），21 d慢性束缚刺激后，第21天T＋D组大鼠血糖比T组较高，差异有显著性（P＜0.01），大鼠5 min内总移动距离有变化，与N组相比，T组差异没有显著性（P＞0.05），T＋D组差异有显著性（P＜0.05）；与N组相比，T组大鼠5 min内移动速度减慢，差异有显著性（P＜0.05），T＋D组差异有显著性（ P＜0.01）。结论利用高脂饲料喂养加腹腔注射小剂量STZ及21天慢性束缚的方法，可以成功复制2型糖尿病兼抑郁症大鼠模型，适用于后续研究。
목적：용체중검측、공복혈당검측、굉관표정、광장실험행위학평개당뇨병겸억욱증적대서모형。방법채용고지사료위양가복강주사소제량련뇨좌균소（ STZ）적방법제비2형당뇨병모형，재기기출상재용21 d만성속박적방법건립당뇨병겸억욱증대서모형。장32지Wistar대서수궤분위3조（ n ＝8）：정상조（N조），2형당뇨병조（T조），2형당뇨병겸억욱증조（T＋D조）。2형당뇨병모형건립후，재만성속박적제0、7、14、21천검측대서적공복혈당화체중，병대대서적굉관표정、음식량、분편、소편、정신상태진행관찰，재제21천이용행위학설비분석연건，대대서광장실험진행분석，검측대서적억욱정도，험증평개2형당뇨병겸억욱증대서모형시부성공。결과급여고지사료급복강주사STZ제비2형당뇨병모형후，T＋D조대서적모발산란，무광택，활동지완，진식량、음수량증가，분편뇨량증가，정신위미。제0、7、14、21천T조화T＋D조조대서체중균하강，여N조비교차이유현저성（P＜0.05；P＜0.01），21d만성속박자격후，T＋D조체중비T조대서체중증가교만，차이유현저성（P＜0.05）；제0、7、14、21천，T조화T＋D조대서혈당균승고，여N조비교차이유현저성（ P＜0.01），21 d만성속박자격후，제21천T＋D조대서혈당비T조교고，차이유현저성（P＜0.01），대서5 min내총이동거리유변화，여N조상비，T조차이몰유현저성（P＞0.05），T＋D조차이유현저성（P＜0.05）；여N조상비，T조대서5 min내이동속도감만，차이유현저성（P＜0.05），T＋D조차이유현저성（ P＜0.01）。결론이용고지사료위양가복강주사소제량STZ급21천만성속박적방법，가이성공복제2형당뇨병겸억욱증대서모형，괄용우후속연구。
Objective To evaluate the rat model of type 2 diabetes mellitus ( T2DM) associated with depres-sion by body weight, fasting blood glucose, manifestations, and open field test.Methods The T2DM rat model was induced by high fat diet and low dose of STZ injection, and in addition, the T2DM rats were made restraint stress for 21 days.32 Wistar rats were randomly divided into three groups: normal group ( group N) , T2DM group ( group T)and T2 DM with depression group ( group T +D) , with 8 rats in each group.After the model was established, to measure the body weight, fasting blood glucose at day 0, 7, 14, and 21, and observe the gross manifestations, drink-ing and diet, feces, urine, and mental state, and test the rat depression by open field test.Results After establish-ment of the T+D rat model, the rats in group T+D showed some symptoms, including messy dark and gloomy hair, slow movement, increasing drinking, diet, feces and urine and mental fatigue.At day 0, 7, 14, and 21, compared with the group N, the body weight of the group T and group T+D were decreased, showing a significant difference ( P<0.05;P<0.01).At day 0, 7, 14, and 21, compared with the group N, and the fasting blood glucose in the groups T and T+D were increased, with a significant difference ( P<0.01 ) .After 21 days of restraint stress, the fasting blood glucose in the group T+D was significantly higher than that in the group T ( P<0.01 ) .Compared with the group N, the total movement distance in 5 minutes in the group T+D was reduced, but without a significant differ-ence (1532.6 ±126.8 cm vs.940.5 ±208.3 cm, P>0.05).Compared with the group N, the movement speed in 5 minutes in the group T was significantly slower than that in the group T , with a significant difference ( 5.1 ±0.4 cm/s vs.2.9 ±0.6 cm/s, P<0.05 ) , and even more slower than that in the group T +D, with a significant differ-ence (5.1 ±0.4 cm/s vs.2.4 ±0.5 cm/s, P<0.01) .Conclusions A rat model of type 2 diabetes mellitus as-sociated with depression has been successfully established by high fat diet and injection of low dose streptozotocin in combination with restraint stress for 21 days.This rat model is useful for further relevant studies.