CAJ | 학술논문

目的：探讨miR-182模拟物对1型糖尿病小鼠心脏功能的影响及可能作用机制。方法40只8周龄雄性C57小鼠随机分为正常对照组（n＝5），miR-182模拟物对照组（n＝5），1型糖尿病组（n＝15），1型糖尿病＋miR-182模拟物治疗组（n＝15）。腹腔注射链脲佐菌素（STZ）建立1型糖尿病动物模型。实验于8周末结束，采用小动物用高分辨超声仪测量小鼠心脏功能；运用透射电镜观察心肌组织超微结构改变；利用real-time PCR技术检测心肌组织β-肌球蛋白重链（β-MHC），α-肌球蛋白重链（α-MHC），心房钠尿肽（ANP），I型（Col I）和III型胶原（Col III） mRNA及miR-182 mRNA的表达含量。结果①miR-182模拟物可改善1型糖尿病小鼠的心脏功能，增加心脏射血分数及左室短轴缩短率（P＜0.01）；②miR-182模拟物可降低糖尿病小鼠心肌组织ANP、Col I、Col III的表达及β/a-MHC比值（P＜0.01）；③miR182模拟物可改善1型糖尿病小鼠心肌超微结构变化，减轻自噬。结论MiR-182模拟物能改善1型糖尿病小鼠的心脏功能，其机制可能与减轻心肌肥大和心肌纤维化，减轻线粒体结构损伤及减轻自噬有关。
목적：탐토miR-182모의물대1형당뇨병소서심장공능적영향급가능작용궤제。방법40지8주령웅성C57소서수궤분위정상대조조（n＝5），miR-182모의물대조조（n＝5），1형당뇨병조（n＝15），1형당뇨병＋miR-182모의물치료조（n＝15）。복강주사련뇨좌균소（STZ）건립1형당뇨병동물모형。실험우8주말결속，채용소동물용고분변초성의측량소서심장공능；운용투사전경관찰심기조직초미결구개변；이용real-time PCR기술검측심기조직β-기구단백중련（β-MHC），α-기구단백중련（α-MHC），심방납뇨태（ANP），I형（Col I）화III형효원（Col III） mRNA급miR-182 mRNA적표체함량。결과①miR-182모의물가개선1형당뇨병소서적심장공능，증가심장사혈분수급좌실단축축단솔（P＜0.01）；②miR-182모의물가강저당뇨병소서심기조직ANP、Col I、Col III적표체급β/a-MHC비치（P＜0.01）；③miR182모의물가개선1형당뇨병소서심기초미결구변화，감경자서。결론MiR-182모의물능개선1형당뇨병소서적심장공능，기궤제가능여감경심기비대화심기섬유화，감경선립체결구손상급감경자서유관。
Objective To investigate the effects of miR-182 mimics on cardiac function in type 1 diabetic mice and explore the mechanisms of action .Methods Forty 8-week-old C57 mice were randomly divided into 4 groups:control group (n=5), miR-182 mimics control groups group (n=5), type 1 diabetes group (n=15) and type 1 diabetes treated by miR-182 mimics (n=15).Eight weeks after modeling , the cardiac function was evaluated by a high-resolution ultra-sonic imaging system for small animals (Vevo 2100).The samples of cardiac tissue was used to observe the ultrastructural changes by electron microscopy and to detect the expression levels of β-MHC,α-MHC, ANP, Col I, Col III and miR-182 mRNA .Results ①MiR-182 mimics improved the cadiac function of type 1 diabetic mice with an increase of left ventricu-lar function and left ventricular short axis fractional shortening ( P<0.01);②MiR-182 mimics significantly decreased the expression level of ANP , Col I, Col III protein and the ratio of β/α-MHC in cardiac tissue;③MiR-182 mimics induced ul-trastructural changes of cardiac tissue in type 1 diabetic mice and the phenomenon of autophagy .Conclusions MiR-182 mimics may improve the cardiac function of type 1 diabetic mice , which is associated with decreased expression of hypertro-phy-related gene ANP,β/α-MHC ratio and expression of Col I, Col III, reduction of mitochondrial damage and autophagy .