检验检疫学刊
檢驗檢疫學刊
검험검역학간
INSPECTION AND QUARANTINE SCIENCE
2014年
4期
58-61,66
,共5页
林二妹%白红岩%孙肖红%徐宝梁
林二妹%白紅巖%孫肖紅%徐寶樑
림이매%백홍암%손초홍%서보량
昆津病毒%巴马哈森林病毒%RT-PCR
昆津病毒%巴馬哈森林病毒%RT-PCR
곤진병독%파마합삼림병독%RT-PCR
Kunjin Virus%Barmah Forest Virus%RT-PCR
[目的]建立昆津病毒和巴马哈森林病毒双重RT-PCR检测方法。[方法]利用Beacon Designer7.0软件设计引物，以人工合成昆津病毒巴马哈森林病毒E基因的片段作为模板，验证该方法的灵敏度及特异性。[结果]最低检出限：昆津病毒1.83×106 copies/μL，巴马哈森林病毒2.02×106copies/μL。[结论]建立的昆津病毒和巴马哈森林病毒双重RT-PCR检测方法具有灵敏度高、特异性好等特点，适合于昆津病毒和巴马哈森林病毒的快速检测。
[目的]建立昆津病毒和巴馬哈森林病毒雙重RT-PCR檢測方法。[方法]利用Beacon Designer7.0軟件設計引物，以人工閤成昆津病毒巴馬哈森林病毒E基因的片段作為模闆，驗證該方法的靈敏度及特異性。[結果]最低檢齣限：昆津病毒1.83×106 copies/μL，巴馬哈森林病毒2.02×106copies/μL。[結論]建立的昆津病毒和巴馬哈森林病毒雙重RT-PCR檢測方法具有靈敏度高、特異性好等特點，適閤于昆津病毒和巴馬哈森林病毒的快速檢測。
[목적]건립곤진병독화파마합삼림병독쌍중RT-PCR검측방법。[방법]이용Beacon Designer7.0연건설계인물，이인공합성곤진병독파마합삼림병독E기인적편단작위모판，험증해방법적령민도급특이성。[결과]최저검출한：곤진병독1.83×106 copies/μL，파마합삼림병독2.02×106copies/μL。[결론]건립적곤진병독화파마합삼림병독쌍중RT-PCR검측방법구유령민도고、특이성호등특점，괄합우곤진병독화파마합삼림병독적쾌속검측。
A duplex RT-PCR method for detection of Kunjin virus (KUNV) and Barmah Forest virus (BFV) was established. Based on the E gene segments of KUNV and BFV respectively, two twin primers were designed and synthesized, and the RT-PCR was developed. The specialty and the sensitivity were tested. The detection limits of the Kunjin virus was 1.83×106 copies/μL, and that of Barmah Forest virus, 2.02×106 copies/μL. A Duplex RT-PCR for KUNV and BFV detection was established and characterized by high sensitivity and rapidity.
