CAJ | 학술논문

[目的]通过彗星电泳法检测样品单细胞DNA损伤从而鉴别辐照食品。[方法]选取12种食品基质，经1KGy、3KGy、5KGy、7KGy、10KGy剂量辐照后，进行彗星电泳检测，并对检测时效性进行研究。[结果]除熟制鸡肉外，其他样品均可通过彗星电泳法检测到辐照状况，检测灵敏度达1KGy辐照剂量；辐照产生的DNA损伤具有明显的剂量-效应关系，以TL、TDNA%指标分析存在平台期，以TM、OTM指标分析则不存在平台期；辐照后6h-5d均可检测到明显的彗星图像。[结论]彗星电泳法适用于除熟制食品外的含DNA食品的辐照检测，方法稳定性好，检测灵敏度高。
[목적]통과혜성전영법검측양품단세포DNA손상종이감별복조식품。[방법]선취12충식품기질，경1KGy、3KGy、5KGy、7KGy、10KGy제량복조후，진행혜성전영검측，병대검측시효성진행연구。[결과]제숙제계육외，기타양품균가통과혜성전영법검측도복조상황，검측령민도체1KGy복조제량；복조산생적DNA손상구유명현적제량-효응관계，이TL、TDNA%지표분석존재평태기，이TM、OTM지표분석칙불존재평태기；복조후6h-5d균가검측도명현적혜성도상。[결론]혜성전영법괄용우제숙제식품외적함DNA식품적복조검측，방법은정성호，검측령민도고。
The single cell DNA damage was detected by using comet assay to screen irradiated foods. In principle, it is applicable to all the detection of irradiated food containing DNA. Twelve food matrixes were irradiated at 1KGy, 3KGy, 5KGy, 7KGy and 10KGy dose respectively and then detected by comet assay. The time effect of the method was also studied. The irradiation of eleven samples, except the cooked chicken, could be identified through comet assay. The detection sensitivity was 1Kgy. DNA damage of irradiation was in obvious dose-effect relationship. Platform period was found by the index TL and TDNA%, other than by TM and OTM. The detection efficiency of the method was identified from six hours to five days. Except cooked food comet assay is suitable for detecting irradiated foods which contains DNA damages. The method has good stability and high sensitivity.