CAJ | 학술논문

目的：检测 ox-LDL 对破骨细胞增殖及分化的影响。方法 CuSO4氧化法制备 ox-LDL，硫代巴比妥法鉴定低密度脂蛋白氧化程度，将制备的 ox-LDL 配置成不同的浓度，对破骨细胞进行干预，MTT 法测定其对破骨细胞增殖及分化的影响。结果不同浓度的 ox-LDL 对破骨细胞的增殖及分化的影响有差别：ox-LDL 浓度分别为100 mg/ L，150 mg/ L，200 mg/ L 时，破骨细胞抑制率为0.65±0.732，0.48±0.068，0.31±0.039，都较对照组破骨细胞的1.28±0.147低，P ﹤0.05。而 ox-LDL 浓度为25 mg/ L 及50mg/ L 时，破骨细胞抑制率为1.134±0.120，1.189±0.112，P ﹥0.05。结论成功制备了氧化性低密度脂蛋白及诱导形成破骨细胞，一定浓度的 ox-LDL 可促进破骨细胞的增殖及分化。
목적：검측 ox-LDL 대파골세포증식급분화적영향。방법 CuSO4양화법제비 ox-LDL，류대파비타법감정저밀도지단백양화정도，장제비적 ox-LDL 배치성불동적농도，대파골세포진행간예，MTT 법측정기대파골세포증식급분화적영향。결과불동농도적 ox-LDL 대파골세포적증식급분화적영향유차별：ox-LDL 농도분별위100 mg/ L，150 mg/ L，200 mg/ L 시，파골세포억제솔위0.65±0.732，0.48±0.068，0.31±0.039，도교대조조파골세포적1.28±0.147저，P ﹤0.05。이 ox-LDL 농도위25 mg/ L 급50mg/ L 시，파골세포억제솔위1.134±0.120，1.189±0.112，P ﹥0.05。결론성공제비료양화성저밀도지단백급유도형성파골세포，일정농도적 ox-LDL 가촉진파골세포적증식급분화。
Objective:To explore the effect of ox-LDL on the proliferation and differentiation of osteoclasts. Methods:ox-LDL was prepareed by copper ion oxidation. Degree of ox-LDL was identified by thiobarbituric. Prepared ox-LDL was configured into different concentrations and added to osteoclasts. The effects on osteoclast proliferation and differentiation were assayed by the method of MTT. Results:The degree of osteoclast proliferation and differentiation differed significantly among the groups of different concentrations of ox-LDL. When ox-LDL concentration was 100mg / L,150mg/ L and 200 mg/ L,osteoclast inhibition rate was 0. 65 ± 0. 732,0. 48 ± 0. 068 and 0. 31 ± 0. 039 respectively,which was significantly lower than those of the control group(1. 28 ± 0. 147,P ﹤ 0. 05). The ox-LDL concentration of 25 mg/ L and 50mg/ L,the osteoclast inhibition rate was 1. 134 ± 0. 120 and 1. 189 ± 0. 112 respectively(P ﹥ 0. 05). Conclusion:A certain con-centration of ox-LDL can promote proliferation and differentiation of osteoclasts.