CAJ | 학술논문

株系紫云英品种的 DNA为模板进行PCR 扩增，筛选出40条扩增条带较好的 ISSR引物，其中有10个引物扩增出的条带多态性较好，从500~3000 bp共扩出684个条带，平均多态率59.2%，表明供试的紫云英品种资源的遗传多样性相依程度较高。采用 NTSYS 2.1软件分析，22个紫云英品种间的相似性系数界于0.63~0.95。 UPGMA法聚类将22个紫云英品种分为4类，研究表明 ISSR分子标记方法可较好的应用于紫云英品种遗传多样性分析上，并为紫云英品种间的鉴定分类提供了分子水平的依据。
주계자운영품충적 DNA위모판진행PCR 확증，사선출40조확증조대교호적 ISSR인물，기중유10개인물확증출적조대다태성교호，종500~3000 bp공확출684개조대，평균다태솔59.2%，표명공시적자운영품충자원적유전다양성상의정도교고。채용 NTSYS 2.1연건분석，22개자운영품충간적상사성계수계우0.63~0.95。 UPGMA법취류장22개자운영품충분위4류，연구표명 ISSR분자표기방법가교호적응용우자운영품충유전다양성분석상，병위자운영품충간적감정분류제공료분자수평적의거。
Genetic relationships among 22 accessions of Astragalus sinicus L. col ect-ed from different provinces of China were analyzed by inter-simple sequence repeat (ISSR) markers. The results showed that 10 highly reproducible ISSR fragments a-mong 40 primers were screened. Using these primers, a total of 684 ISSR frag-ments from 500 to 3 000 bp were amplified, and 59.2% of them showed polymor-phic by unweighted pair-group method with arithmetic (UPGMA) analysis. It revealed that the 22 accessions had a similarity range from 0.63 to 0.95, and existed biolog-ical diversities. Based on cluster and principal coordinate analyses, al accessions could be divided into four distinct groups.