农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
8期
1274-1276,1287
,共4页
巴西木%刺盘孢%炭疽病%ITS序列分析
巴西木%刺盤孢%炭疽病%ITS序列分析
파서목%자반포%탄저병%ITS서렬분석
Dracaena fragrans%Col etotrichum%Anthracnose%ITS sequence analysis
[目的]对巴西木炭疽病病原菌进行鉴定及 rDNA鄄ITS序列分析。[方法]该文对巴西木炭疽病病原菌进行分离、形态学观察。通过 rDNA鄄ITS的扩增与序列测定，对 rDNA鄄ITS进行序列分析。[结果]该菌分生孢子直或弯，椭圆形至新月形，2-5个油滴，7.5-20×4.5-5μm。经ITS系统学分析，确定该试验分离到的刺盘孢为一新种，并命名为 Colletotrichum dracaena鄄fragrantis sp. novA。[结论]为炭疽病防止研究提供理论依据。
[目的]對巴西木炭疽病病原菌進行鑒定及 rDNA鄄ITS序列分析。[方法]該文對巴西木炭疽病病原菌進行分離、形態學觀察。通過 rDNA鄄ITS的擴增與序列測定，對 rDNA鄄ITS進行序列分析。[結果]該菌分生孢子直或彎，橢圓形至新月形，2-5箇油滴，7.5-20×4.5-5μm。經ITS繫統學分析，確定該試驗分離到的刺盤孢為一新種，併命名為 Colletotrichum dracaena鄄fragrantis sp. novA。[結論]為炭疽病防止研究提供理論依據。
[목적]대파서목탄저병병원균진행감정급 rDNA견ITS서렬분석。[방법]해문대파서목탄저병병원균진행분리、형태학관찰。통과 rDNA견ITS적확증여서렬측정，대 rDNA견ITS진행서렬분석。[결과]해균분생포자직혹만，타원형지신월형，2-5개유적，7.5-20×4.5-5μm。경ITS계통학분석，학정해시험분리도적자반포위일신충，병명명위 Colletotrichum dracaena견fragrantis sp. novA。[결론]위탄저병방지연구제공이론의거。
In this study, identification and rDNA-ITS sequence analysis of an anthracnose pathogen on Dracaena fragrans were carried out. [Method] D. fra-grans leaves with lesions were used as experimental materials to isolate anthrac-nose pathogen. Morphological observation, rDNA-ITS amplification and sequence analysis were performed to identify the pathogen strain. [Result] Caonidia of the iso-lated anthracnose pathogen were straight or curved, el iptic to crescent, with 2-5 oil droplets, 7.5-20 × 4.5-5 μm. According to molecular phylogenetic analysis, the iso-lated pathogen strain was identified as a new species, which was named Col-letotrichum dracaena-fragrantis sp. nov. [Conclusion] This study provided theoretical basis for the prevention and control of anthracnose.
