CAJ | 학술논문

目的：研究用毛细管气相色谱法测定牛奶中甜蜜素含量的分析方法，通过对沉降剂用量、酯化时间等条件的优化，降低方法的检出限。方法：利用甜蜜素（环己基氨基磺酸钠）在硫酸介质中与亚硝酸钠反应后生成环己醇亚硝酸酯，用正己烷萃取，并用气相色谱检测环己醇亚硝酸酯的含量。结果：甜蜜素的线性范围为0.05~0.4 mg/mL，方法检出限为2μg，加标回收率在99.4%~102.5%之间，RSD为1.4%。结论：该方法简便、快捷、准确度高、重现性好，方法检出限低，优于GB/T5009.97-2003法的4μg检出限。
목적：연구용모세관기상색보법측정우내중첨밀소함량적분석방법，통과대침강제용량、지화시간등조건적우화，강저방법적검출한。방법：이용첨밀소（배기기안기광산납）재류산개질중여아초산납반응후생성배기순아초산지，용정기완췌취，병용기상색보검측배기순아초산지적함량。결과：첨밀소적선성범위위0.05~0.4 mg/mL，방법검출한위2μg，가표회수솔재99.4%~102.5%지간，RSD위1.4%。결론：해방법간편、쾌첩、준학도고、중현성호，방법검출한저，우우GB/T5009.97-2003법적4μg검출한。
Objective:To study the analysis method of capillary gas chromatography in determination of sodium cyclamate content in milk and to lower the detection limit of the method by optimizing the settling agent dosage, esterification time and other conditions. Methods: Sodium cyclamate reacted in the medium of sulfuric acid with sodium nitrite and cyclohexanol nitrite was produced and extracted with n-hexane, and its content was determined by capillary gas chromatography. Results: Linear range was from 0.05 to 0.4 mg/mL, the detection limit was 2 μg, recovery of standard addition was 99.4%-102.5%, and RSD was 1.4%. Conclusion: The method was simple, rapid and accurate, with good reproducibility and low detection limit, much better than detection limit of 4μg by GB/T5009.97-2003 method.