CAJ | 학술논문

目的：初步摸索出一条适合工业化开发、高效的、可溶表达结核分枝杆菌MTB8.1蛋白的发酵工艺。方法：利用菌株pUC18/MTB8.1/DH10B为发酵株，通过对培养基组成和发酵参数进行系统研究，从而获得一全新的发酵工艺。结果：重组结核分枝杆菌MTB8.1蛋白通过本发酵工艺的表达，单位体积发酵液可溶性蛋白的产量大幅提高，每升发酵液纯化得到可溶性蛋白约30 mg左右。结论：初步摸索出一条较好的MTB8.1在大肠埃希菌系统中的可溶性表达的途径，为其工业化生产奠定了理论基础。
목적：초보모색출일조괄합공업화개발、고효적、가용표체결핵분지간균MTB8.1단백적발효공예。방법：이용균주pUC18/MTB8.1/DH10B위발효주，통과대배양기조성화발효삼수진행계통연구，종이획득일전신적발효공예。결과：중조결핵분지간균MTB8.1단백통과본발효공예적표체，단위체적발효액가용성단백적산량대폭제고，매승발효액순화득도가용성단백약30 mg좌우。결론：초보모색출일조교호적MTB8.1재대장애희균계통중적가용성표체적도경，위기공업화생산전정료이론기출。
Objective: To explore an effective fermentation process of Mycobacterium tuberculosis protein MTB8.1 with soluble expression, which would be suitable for industrial development. Methods: Strain pUC18/MTB8.1/DH10B was used as fermentation strain and a systematic study of the composition of medium and the parameters of fermentation was conducted to obtain a new fermentation process. Results:By means of the new fermentation process, the yield of soluble recombinant protein MTB8.1 was greatly increased in per unit volume of fermentation broth, with about 30 mg of soluble protein obtained from every liter of fermentation broth through purification. Conclusion: A better way of soluble expression of MTB8.1 in Escherichia coli expression system was found tentatively, which laid the theoretical foundation for the industrial production.