CAJ | 학술논문

目的：研究美洲大蠊提取物（CⅡ-3）对ISO所致大鼠心肌缺血的保护作用。方法：采用皮下注射ISO诱发大鼠心肌缺血。观察心肌缺血大鼠ECG的S-T段、Q-T间期的变化，血清中LDH、SOD、CK和MDA含量及心肌病理损伤情况。结果：3个剂量组的CⅡ-3都能降低S-T段的偏移和缩短Q-T间期（P<0.05），中、高剂量组能显著降低MDA的含量（P<0.01）和提高SOD的活性（P<0.05），高剂量组能降低CK的活性（P<0.05），但3个剂量组对LDH活性影响较小（P>0.05）。病理检测显示3个剂量组的CⅡ-3能不同程度的减轻大鼠心肌病理组织的损伤程度。结论：CⅡ-3对ISO诱导的大鼠心肌缺血具有一定的保护作用。
목적：연구미주대렴제취물（CⅡ-3）대ISO소치대서심기결혈적보호작용。방법：채용피하주사ISO유발대서심기결혈。관찰심기결혈대서ECG적S-T단、Q-T간기적변화，혈청중LDH、SOD、CK화MDA함량급심기병리손상정황。결과：3개제량조적CⅡ-3도능강저S-T단적편이화축단Q-T간기（P<0.05），중、고제량조능현저강저MDA적함량（P<0.01）화제고SOD적활성（P<0.05），고제량조능강저CK적활성（P<0.05），단3개제량조대LDH활성영향교소（P>0.05）。병리검측현시3개제량조적CⅡ-3능불동정도적감경대서심기병리조직적손상정도。결론：CⅡ-3대ISO유도적대서심기결혈구유일정적보호작용。
Objective:To study the protective effect of CⅡ-3 extracted from Periplaneta americana on myocardial ischemia in rats induced by isoproterenol (ISO). Methods:The myocardial ischemia model was made by using subcutaneous injection of ISO in rats. The change of S-T segment and Q-T interval of electrocardiogram (ECG) was observed, and the content of LDH, CK, SOD and MDA in serum was measured. The pathological lesion of myocardium were observed. Results:Three types of dosage of CⅡ-3(low, 25 mg/kg;mid, 50 mg/kg;high, 100 mg/kg)all significantly reduced S-T segment deviation and shortened Q-T interval(P<0. 05). Middle and high dosage of CⅡ-3 significantly reduced the content of MDA( P < 0. 01) and raised the activity of SOD (P < 0. 05). High dosage of CⅡ-3 reduced the activity of CK (P < 0. 05), but three dosage of CⅡ-3 had the subtle effect on the activity of LDH. Pathological examination showed that three dosages of CⅡ-3 could reduce the pathological lesion of myocardium in rats to varying degrees. Conclusion:CⅡ-3 had some protective effect on myocardial ischemia in rats induced by ISO.