作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2014年
9期
1702-1709
,共8页
曹红利%岳川%周艳华%王璐%郝心愿%杨亚军%王新超
曹紅利%嶽川%週豔華%王璐%郝心願%楊亞軍%王新超
조홍리%악천%주염화%왕로%학심원%양아군%왕신초
茶树%bZIP转录因子%CsbZIP1%亚细胞定位%克隆表达
茶樹%bZIP轉錄因子%CsbZIP1%亞細胞定位%剋隆錶達
다수%bZIP전록인자%CsbZIP1%아세포정위%극륭표체
Tea plant (Camellia sinensis)%bZIP transcription factor%CsbZIP1%Subcellular localization%Cloning and expression
碱性亮氨酸拉链蛋白(bZIP)作为真核生物中分布最广、最保守的一类转录因子,参与多种生物学过程,尤其在植物抵御各种逆境胁迫中有重要作用。采用RACE和RT-PCR技术克隆到茶树bZIP转录因子基因全长cDNA序列,命名为CsbZIP1(GenBank登录号为JX050148.1)。该基因cDNA全长1515 bp,包含813 bp的完整开放阅读框(ORF),编码270个氨基酸,预测分子量29.484 kD；含有bZIP家族典型的BRLZ结构域碱性结构域和亮氨酸拉链,属于B-zip1家族；系统发育树分析显示CsbZIP1属于bZIP转录因子F亚家族；亚细胞定位结果表明CsbZIP1主要定位于细胞核；qRT-PCR分析表明,4℃低温和NaCl盐胁迫处理均能诱导CsbZIP1的表达,表达量变化趋势都是随着胁迫时间先逐渐升高,到24 h时降低, ABA胁迫处理24 h抑制CsbZIP1的表达。推测CsbZIP1与茶树低温、盐等逆境胁迫密切相关。
堿性亮氨痠拉鏈蛋白(bZIP)作為真覈生物中分佈最廣、最保守的一類轉錄因子,參與多種生物學過程,尤其在植物牴禦各種逆境脅迫中有重要作用。採用RACE和RT-PCR技術剋隆到茶樹bZIP轉錄因子基因全長cDNA序列,命名為CsbZIP1(GenBank登錄號為JX050148.1)。該基因cDNA全長1515 bp,包含813 bp的完整開放閱讀框(ORF),編碼270箇氨基痠,預測分子量29.484 kD；含有bZIP傢族典型的BRLZ結構域堿性結構域和亮氨痠拉鏈,屬于B-zip1傢族；繫統髮育樹分析顯示CsbZIP1屬于bZIP轉錄因子F亞傢族；亞細胞定位結果錶明CsbZIP1主要定位于細胞覈；qRT-PCR分析錶明,4℃低溫和NaCl鹽脅迫處理均能誘導CsbZIP1的錶達,錶達量變化趨勢都是隨著脅迫時間先逐漸升高,到24 h時降低, ABA脅迫處理24 h抑製CsbZIP1的錶達。推測CsbZIP1與茶樹低溫、鹽等逆境脅迫密切相關。
감성량안산랍련단백(bZIP)작위진핵생물중분포최엄、최보수적일류전록인자,삼여다충생물학과정,우기재식물저어각충역경협박중유중요작용。채용RACE화RT-PCR기술극륭도다수bZIP전록인자기인전장cDNA서렬,명명위CsbZIP1(GenBank등록호위JX050148.1)。해기인cDNA전장1515 bp,포함813 bp적완정개방열독광(ORF),편마270개안기산,예측분자량29.484 kD；함유bZIP가족전형적BRLZ결구역감성결구역화량안산랍련,속우B-zip1가족；계통발육수분석현시CsbZIP1속우bZIP전록인자F아가족；아세포정위결과표명CsbZIP1주요정위우세포핵；qRT-PCR분석표명,4℃저온화NaCl염협박처리균능유도CsbZIP1적표체,표체량변화추세도시수착협박시간선축점승고,도24 h시강저, ABA협박처리24 h억제CsbZIP1적표체。추측CsbZIP1여다수저온、염등역경협박밀절상관。
The basic leucine zipper proteins (bZIP) are one of the most extensive and conserved transcriptional factors families in eukaryotes, and plant bZIPs play important roles in many biological processes, especialy for resisting abiotic stresses. In this study, a bZIP full-length cDNA sequence was cloned using RACE and reverse transcription-PCR(RT-PCR) techniques from tea plant (Camellia sinensis). The obtained full-length cDNA was named CsbZIP1 with GenBank accession number JX050148.1. It is 1515 bp in length, containing a 813 bp open reading frame (ORF), encoding 270 amino acid residues with 29.484 kD molecular weight, and containing a typical BRLZ motif (basic region domain and leucine zipper domain) of B-zip1 family. The phylogenetic tree analysis revealed that CsbZIP1 belongs to F subfamily of bZIP. The subcellular location showed that CsbZIP1 protein is located in nucleus. The qRT-PCR analysis indicated that the expression level of CsbZIP1 was up-regulated by cold (4℃) and salt (NaCl) treatments, and both expression amounts increased at first, then declined after 24 hours. However, the expression pattern was down-regulated by ABA treatment within 24 hours. These results demonstrated that CsbZIP1 could be associated with cold and salt stresses in tea plant.
