作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2014年
9期
1619-1628
,共10页
罗宝杰%许俊旭%丁艳锋%李刚华%刘正辉%王绍华
囉寶傑%許俊旭%丁豔鋒%李剛華%劉正輝%王紹華
라보걸%허준욱%정염봉%리강화%류정휘%왕소화
水稻%分蘖芽%休眠与萌发%激素平衡
水稻%分蘗芽%休眠與萌髮%激素平衡
수도%분얼아%휴면여맹발%격소평형
Rice%Tiller bud%Dormancy and germination%Endogenous hormone balance
研究水稻抽穗期分蘖休眠与萌发转换过程中CTK和IAA含量及CTK/IAA值的变化,探讨内源激素平衡对水稻分蘖的调控作用。采用外施6-BA 打破分蘖芽休眠和去穗后外施 IAA 抑制分蘖芽萌发两种处理,创建分蘖芽休眠与萌发转换的材料,测定伸长节和伸长节分蘖芽中 CTK 和 IAA 的含量。6-BA 处理打破了分蘖芽的休眠,显著提高了倒二节和倒二芽中CTK含量,降低了倒二节中IAA含量,对倒二芽中IAA含量影响不大,显著提高了节、芽中CTK/IAA值。去穗后外施IAA逆转了去穗对节、芽CTK含量提高效果, CTK含量维持在较低值,显著提高了倒二节中IAA含量,对倒二芽中IAA含量无显著影响,显著降低了节、芽中CTK/IAA值。CTK和IAA共同调控分蘖发生,较高的CTK/IAA值利于分蘖芽萌发,较低的CTK/IAA值使其休眠,节中CTK/IAA值在2.6~2.8,芽中CTK/IAA值在4.8~5.0,可作为分蘖芽休眠与萌发转换的临界指标。
研究水稻抽穗期分蘗休眠與萌髮轉換過程中CTK和IAA含量及CTK/IAA值的變化,探討內源激素平衡對水稻分蘗的調控作用。採用外施6-BA 打破分蘗芽休眠和去穗後外施 IAA 抑製分蘗芽萌髮兩種處理,創建分蘗芽休眠與萌髮轉換的材料,測定伸長節和伸長節分蘗芽中 CTK 和 IAA 的含量。6-BA 處理打破瞭分蘗芽的休眠,顯著提高瞭倒二節和倒二芽中CTK含量,降低瞭倒二節中IAA含量,對倒二芽中IAA含量影響不大,顯著提高瞭節、芽中CTK/IAA值。去穗後外施IAA逆轉瞭去穗對節、芽CTK含量提高效果, CTK含量維持在較低值,顯著提高瞭倒二節中IAA含量,對倒二芽中IAA含量無顯著影響,顯著降低瞭節、芽中CTK/IAA值。CTK和IAA共同調控分蘗髮生,較高的CTK/IAA值利于分蘗芽萌髮,較低的CTK/IAA值使其休眠,節中CTK/IAA值在2.6~2.8,芽中CTK/IAA值在4.8~5.0,可作為分蘗芽休眠與萌髮轉換的臨界指標。
연구수도추수기분얼휴면여맹발전환과정중CTK화IAA함량급CTK/IAA치적변화,탐토내원격소평형대수도분얼적조공작용。채용외시6-BA 타파분얼아휴면화거수후외시 IAA 억제분얼아맹발량충처리,창건분얼아휴면여맹발전환적재료,측정신장절화신장절분얼아중 CTK 화 IAA 적함량。6-BA 처리타파료분얼아적휴면,현저제고료도이절화도이아중CTK함량,강저료도이절중IAA함량,대도이아중IAA함량영향불대,현저제고료절、아중CTK/IAA치。거수후외시IAA역전료거수대절、아CTK함량제고효과, CTK함량유지재교저치,현저제고료도이절중IAA함량,대도이아중IAA함량무현저영향,현저강저료절、아중CTK/IAA치。CTK화IAA공동조공분얼발생,교고적CTK/IAA치리우분얼아맹발,교저적CTK/IAA치사기휴면,절중CTK/IAA치재2.6~2.8,아중CTK/IAA치재4.8~5.0,가작위분얼아휴면여맹발전환적림계지표。
The objection of this experiment was to study the changes of contents of CTK, IAA and the ratio of CTK/IAA during heading stage, and to investigate the regulation of endogenous hormones in rice tiller. Exogenous 6-BA and IAA were used to regulate the development of tiller buds, establish the transfer between dormancy and germination, and measure the contents of CTK and IAA in elongated nodes and tiller buds located at the elongated nodes. The exogenous 6-BA increased the CTK contents in nodes and tiller buds, decreased the IAA content in nodes had little effect in the content of IAA in tiller buds, significantly in-creased ratios of CTK/IAA in both nodes and tiller buds, and promoted the transfer from dormancy to germination. External IAA after removing panicle decreased CTK contents in nodes and tiller buds, which were maintained at a low level, increased the IAA content in buds, and significantly decreased the ratio of CTK/IAA in nodes and tiller buds, causing the transfer from germination to dormancy. Tiller development is determined by the balance between CTK and IAA, higher CTK/IAA ratio is benefit to the transfer from dormancy to germination, while lower CTK/IAA ratio inhibits the transfer. The ratios of CTK/IAA at 2.6-2.8 in nodes, 4.8-5.0 in tiller buds can be considered as the critical exponent of the transfer between dormancy and germination.