CAJ | 학술논문

F-box蛋白参与细胞周期调控、细胞凋亡及信号转导等多种生命活动,对维持植物正常生长发育a和介导非生物胁迫响应等过程发挥重要作用。谷子具有显著的耐旱耐瘠薄等特性,本研究根据谷子转录组分析结果,从525个F-box家族成员中鉴定出19个在干旱胁迫下表达量上调的F-box基因,根据序列相似性将其分为6类,同一类基因具有相似的内含子-外显子结构。染色体定位分析发现,这些基因分别分布在谷子的8条染色体上,其中,第2条染色体上含F-box基因最多,有6个。结构域分析结果表明,19个F-box蛋白均含保守的F-box结构域,而C端含FBD、WD40、FBA、ZnF、Kelch和LRR等结构域。启动子元件分析表明,谷子19个F-box基因均含逆境应答元件,其中, MYB和MYC元件的数量最多(9~78个),说明这些基因对干旱应答反应可能主要受MYB、MYC转录因子调控。转录组分析结果表明, SiF-box18基因对干旱胁迫的响应远远高于其他F-box成员,对干旱、高盐、ABA、SA和JA都有响应；亚细胞定位结果显示, SiF-box18蛋白定位在细胞核中。本研究为进一步深入了解SiF-box18基因的功能提供了依据。
F-box단백삼여세포주기조공、세포조망급신호전도등다충생명활동,대유지식물정상생장발육a화개도비생물협박향응등과정발휘중요작용。곡자구유현저적내한내척박등특성,본연구근거곡자전록조분석결과,종525개F-box가족성원중감정출19개재간한협박하표체량상조적F-box기인,근거서렬상사성장기분위6류,동일류기인구유상사적내함자-외현자결구。염색체정위분석발현,저사기인분별분포재곡자적8조염색체상,기중,제2조염색체상함F-box기인최다,유6개。결구역분석결과표명,19개F-box단백균함보수적F-box결구역,이C단함FBD、WD40、FBA、ZnF、Kelch화LRR등결구역。계동자원건분석표명,곡자19개F-box기인균함역경응답원건,기중, MYB화MYC원건적수량최다(9~78개),설명저사기인대간한응답반응가능주요수MYB、MYC전록인자조공。전록조분석결과표명, SiF-box18기인대간한협박적향응원원고우기타F-box성원,대간한、고염、ABA、SA화JA도유향응；아세포정위결과현시, SiF-box18단백정위재세포핵중。본연구위진일보심입료해SiF-box18기인적공능제공료의거。
F-box proteins play critical roles in plant growth and development and respond to abiotic stresses, involving in cell-cycle regulation, cell apoptosis and signal transduction. Foxtail millet has a character with drought-tolerance. We identified 19 up-regulated F-box genes from 525 F-box families under stress in foxtail millet based on the transcriptome sequencing and di-vided them into six subgroups, and the genes in the same subgroup had the same intron-exon structure. These genes were un-evenly distributed on eight foxtail millet chromosomes, and there were six genes on chromosome 2, that gene number was more than that contained on other chromosomes. The analysis of conserved domain of 19 genes showed that all the protein sequences had a conserved F-box motif, and several other domain also existed in C-terminal including FBD, WD40, FBA, ZnF, and Kelch LRR. Putative cis-acting elements showed that the number of MYB and MYC was the most, which ranged from 9 to 78. Tran-scriptome sequencing showed that SiF-box18 was more hypersensitive to the drought stress than other F-box members and re-sponse to drought, salt, ABA, GA, SA, and JA based on the real time PCR. The transient expression in Arabidopsis protoplasts displayed that SiF-box18 protein was localized in nuclear section. This study provided experimental data for further studying the functions of the SiF-box18 gene.