CAJ | 학술논문

结缕草是优良的暖季型草坪草之一，主要用于亚热带和热带地区的草坪种植。抗冷性是结缕草栽培范围的限制因子。本研究以日本最北部原产的结缕草品系为材料，根据其他植物的已知的抗寒基因 CBF 序列，通过同源克隆的方法获得结缕草中相对应的同源基因ZjCBF；根据和其他已报告的CBF序列的比对结果，确定ZjCBF基因属于CBF转录因子家族基因中CBF1型基因。利用半定量PCR和实时定量PCR分析该基因在寒冷条件下的表达情况，发现ZjCBF基因受冷胁迫的诱导，在4℃处理6 h时表达量最高。在此基础上，本研究构建了该基因的过表达载体，并将其转化到拟南芥中，通过低温冷处理实验发现，不论是否经过冷驯化，转 ZjCBF 基因植株由于 ZjCBF 的过量表达均比野生型植株抗寒性强。
결루초시우량적난계형초평초지일，주요용우아열대화열대지구적초평충식。항랭성시결루초재배범위적한제인자。본연구이일본최북부원산적결루초품계위재료，근거기타식물적이지적항한기인 CBF 서렬，통과동원극륭적방법획득결루초중상대응적동원기인ZjCBF；근거화기타이보고적CBF서렬적비대결과，학정ZjCBF기인속우CBF전록인자가족기인중CBF1형기인。이용반정량PCR화실시정량PCR분석해기인재한랭조건하적표체정황，발현ZjCBF기인수랭협박적유도，재4℃처리6 h시표체량최고。재차기출상，본연구구건료해기인적과표체재체，병장기전화도의남개중，통과저온랭처리실험발현，불론시부경과랭순화，전 ZjCBF 기인식주유우 ZjCBF 적과량표체균비야생형식주항한성강。
Zoysiagrass is recognized as an excellent warm-season turfgrass and mainly used in subtropical and tropical regions. Cold stress is a major constraint factor for the cultivation of zoysiagrass. In this study, according to the sequences of cold tolerance gene CBF had been reported in other plant species, we cloned the corresponding homologous of the ZjCBF gene by homology cloning method in Zoysia japonica using a material originated from the most northern area of Japan. Based on the alignment re-sults compared with other reported CBF genes, we found the ZjCBF gene belongs to the CBF1 familiy. By semi-quantitative PCR and real-time quantitative PCR, we analyzed the expression level of the ZjCBF gene in the cold condition and found that ZjCBF was induced by cold stress, and the ZjCBF expression reached peak at six hours post 4°C treatment. In addition, we also con-structed ZjCBF over expression vector and generated transgenic Arabidopsis plants, with better cold tolerance than the wild-type, whether through cold acclimation or not.