CAJ | 학술논문

MYB类转录因子是植物转录因子最大的家族之一,参与控制植物腺毛细胞的模式和形态建成。本研究利用雷蒙德氏棉(Gossypium raimondii) D5基因组数据库以 AtMYB0(GL1, NM_113708)蛋白为参比序列获得同源基因GrMYB0,从徐州142中克隆了陆地棉的GhMYB0,其开放阅读框长度为843 bp,编码280个氨基酸。经过保守结构域分析和亚细胞定位确定GhMYB0为R2R3-MYB转录因子。qRT-PCR的结果表明, GhMYB0在徐州142开花当天开始高调表达,开花后20 d表达量达高峰；在所有的组织器官中,花中表达量最高,其次为胚珠。转基因功能分析结果表明,在野生型拟南芥(Columbia)中过表达 GhMYB0,使其叶片表皮毛与野生型相比明显减少；该基因在拟南芥突变体 gl-1中过表达,能恢复表皮毛缺失型突变体的表型,说明该基因可能对拟南芥表皮毛的形态建成发挥一定作用,本试验为研究R2R3-MYB转录因子在棉纤维起始和伸长过程中的调控作用提供有力证据。
MYB류전록인자시식물전록인자최대적가족지일,삼여공제식물선모세포적모식화형태건성。본연구이용뢰몽덕씨면(Gossypium raimondii) D5기인조수거고이 AtMYB0(GL1, NM_113708)단백위삼비서렬획득동원기인GrMYB0,종서주142중극륭료륙지면적GhMYB0,기개방열독광장도위843 bp,편마280개안기산。경과보수결구역분석화아세포정위학정GhMYB0위R2R3-MYB전록인자。qRT-PCR적결과표명, GhMYB0재서주142개화당천개시고조표체,개화후20 d표체량체고봉；재소유적조직기관중,화중표체량최고,기차위배주。전기인공능분석결과표명,재야생형의남개(Columbia)중과표체 GhMYB0,사기협편표피모여야생형상비명현감소；해기인재의남개돌변체 gl-1중과표체,능회복표피모결실형돌변체적표형,설명해기인가능대의남개표피모적형태건성발휘일정작용,본시험위연구R2R3-MYB전록인자재면섬유기시화신장과정중적조공작용제공유력증거。
MYB transcription factor, one of the most important protein families in plants, is involved in the regulation of secon-dary metabolism, morphogenesis of plant, responding to environment stress and plant hormone. In this study, we used D5 genomic bank of Gossypium raimondii as the reference to AtMYB0 (GL1, NM_113708) protein, and cloned the full-length cDNA of a new MYB transcription factor gene GhMYB0 from cotton (Gossypium hirsutum L.). The open reading frame of GhMYB0 is 843 bp in length, which encodes 280 amino acid residues. GhMYB0 was confirmed as R2R3-MYB transcription factor via conserved struc-ture analysis and subcellular localization. The qRT-PCR result indicated that GhMYB0 was highly expressed at the blossom day, its expresssion amount reached the peak after 20 days, with the most amount in flower, then in ovules. Transgenic funtion analysis indicated that GhMYB0 over-expressed in Arabidopsis lines, showing fewer trichomes in leaf epiderm than in that of the wild type, which suggests that the gene can restore the notrichome phenotype of gl-1 mutant of Arabidopsis. In addition, the transgenic lines had shorter plant heights, longer vegetative growth time, and pollen abortion.