CAJ | 학술논문

利用高效液相色谱法测定鲜食葡萄采后贮藏过程中糖分的变化．色谱柱为Inertsil NH2柱，流动相为75%的乙腈水溶液，用示差折光检测器检测不同贮藏期鲜食葡萄果实中的糖分．本方法的相对标准偏差为0.52％～1.22％，相关系数分别为0.9973和0.9993，加标回收率为99.81％～100.4％．该方法简便、灵敏、结果可靠，两种糖都得到了较好的分离，适用于鲜食葡萄果实中糖分的分析.
이용고효액상색보법측정선식포도채후저장과정중당분적변화．색보주위Inertsil NH2주，류동상위75%적을정수용액，용시차절광검측기검측불동저장기선식포도과실중적당분．본방법적상대표준편차위0.52％～1.22％，상관계수분별위0.9973화0.9993，가표회수솔위99.81％～100.4％．해방법간편、령민、결과가고，량충당도득도료교호적분리，괄용우선식포도과실중당분적분석.
Determination of saccharide components in table grape was studied by high performance liquid chromatography (HPLC).The HPLC column is Inertsil NH2, 5 μm, 4.6×250 mm,the mobile phase is CH3CN: H2O=75:25, and the detector is series RID-10A refractive index detector.The relative standard deviations are 0.52%~1.22%, and recoveries ranged from 99.81~100.4%, while the linear related coefficients are 0.997 3 and 0.999 3, respectively.The results showed that the samples are separated completely.The method is proved to be simple,rapid and reliable to determine the saccharine components in table grape.