肿瘤药学
腫瘤藥學
종류약학
ANTI-TUMOR PHARMACY
2014年
4期
263-266
,共4页
蒋娟%邹勇德%蒋福平%陈力
蔣娟%鄒勇德%蔣福平%陳力
장연%추용덕%장복평%진력
IL-37%HeLa细胞%细胞增殖%细胞凋亡
IL-37%HeLa細胞%細胞增殖%細胞凋亡
IL-37%HeLa세포%세포증식%세포조망
IL-37%HeLa cells%Cell proliferation%Apoptosis
目的：探讨炎症抑制因子IL-37对宫颈癌HeLa细胞株增殖和凋亡的影响。方法将携带有目的基因IL-37的过表达载体转染入体外培养的HeLa细胞，用MTT法检测细胞的增殖情况，流式细胞仪测定细胞周期及凋亡率，以野生型HeLa细胞以及转染空载体的HeLa细胞作为阴性对照和空载体对照。结果荧光显微镜观察结果显示IL-37质粒成功转染HeLa细胞。MTT检测结果显示，转染后12 h至96 h，转染组OD值明显低于空载体对照组（P<0.01），而空载体对照组和阴性对照组的OD值无明显差异；流式细胞仪检测测结果显示，转染组细胞早期凋亡率较空载体对照组显著升高（P<0.05），而空载体对照组和阴性对照组的早期凋亡率无显著性差异。结论过表达IL-37的HeLa细胞株构建成功。IL-37可抑制宫颈癌HeLa的增殖并诱导其凋亡。
目的：探討炎癥抑製因子IL-37對宮頸癌HeLa細胞株增殖和凋亡的影響。方法將攜帶有目的基因IL-37的過錶達載體轉染入體外培養的HeLa細胞，用MTT法檢測細胞的增殖情況，流式細胞儀測定細胞週期及凋亡率，以野生型HeLa細胞以及轉染空載體的HeLa細胞作為陰性對照和空載體對照。結果熒光顯微鏡觀察結果顯示IL-37質粒成功轉染HeLa細胞。MTT檢測結果顯示，轉染後12 h至96 h，轉染組OD值明顯低于空載體對照組（P<0.01），而空載體對照組和陰性對照組的OD值無明顯差異；流式細胞儀檢測測結果顯示，轉染組細胞早期凋亡率較空載體對照組顯著升高（P<0.05），而空載體對照組和陰性對照組的早期凋亡率無顯著性差異。結論過錶達IL-37的HeLa細胞株構建成功。IL-37可抑製宮頸癌HeLa的增殖併誘導其凋亡。
목적：탐토염증억제인자IL-37대궁경암HeLa세포주증식화조망적영향。방법장휴대유목적기인IL-37적과표체재체전염입체외배양적HeLa세포，용MTT법검측세포적증식정황，류식세포의측정세포주기급조망솔，이야생형HeLa세포이급전염공재체적HeLa세포작위음성대조화공재체대조。결과형광현미경관찰결과현시IL-37질립성공전염HeLa세포。MTT검측결과현시，전염후12 h지96 h，전염조OD치명현저우공재체대조조（P<0.01），이공재체대조조화음성대조조적OD치무명현차이；류식세포의검측측결과현시，전염조세포조기조망솔교공재체대조조현저승고（P<0.05），이공재체대조조화음성대조조적조기조망솔무현저성차이。결론과표체IL-37적HeLa세포주구건성공。IL-37가억제궁경암HeLa적증식병유도기조망。
Objective To investigate the effect of inflammation inhibitor IL-37 on the growth and apoptosis of cervical cancer cell line HeLa. Methods The shuttle vector pAd track CMV that carried the objective gene of IL-37 was transfected into HeLa cells. Effects of IL-37 on growth and apoptosis of the transfected cells were evaluated by Methyl thiazolyl tetrazo-lium (MTT) rapid photocolorimetric assay and flow cytometry (FCM). At the same time, pAd track CMV was transfected into HeLa cells as empty vector control while wild type HeLa cells as negative control. Results Fluorescence microscopy showed that IL-37 plasmid was successfully transfected into HeLa cells. MTT assay showed that 12 h~96 h after transfection, the OD of IL-37 transfected HeLa cells was significantly lower than the empty vector control group (P<0.01). But the OD showed no significant difference between the empty vector control group and negative control group had no significant dif-ference;FCM results showed that the early apoptosis rate of IL-37 transfected HeLa cells was significantly higher than that of the empty vector control group (P<0.05), while the empty vector control group and negative control group. Conclusion The IL-37 overexpressed HeLa cell line was successfully constructed. IL-37 can inhibit proliferation and induce apoptosis of HeLa cells.
