CAJ | 학술논문

目的：探讨上皮型钙黏附素（E-cadherin）、神经型钙黏附素（N-cadherin）及Snail在非小细胞肺癌（NSCLC）中的表达及其临床意义。方法采用实时荧光定量PCR测定Snail、E-cadherin和N-cadherin基因在10对非小细胞肺癌及其癌旁正常肺组织中的表达情况，同时采用免疫组化法检测105例NSCLC以及41例癌旁组织中Snail、E-cadherin和N-cadherin蛋白的表达。结果与癌旁正常肺组织相比较，非小细胞肺癌组织中E-cadherin的表达显著减少，N-cadherin和Snail表达明显增加（P<0.05）。NSCLC组织中，Snail的表达与N-cadherin的表达呈明显的正相关（P<0.05，r=0.21），与E-cadherin的表达呈显著负相关（P<0.05，r=-0.39），而N-cadherinl的表达与E-cadherin的表达也呈明显负相关（P<0.05，r=-0.53）。结论非小细胞肺癌组织中， Snail、N-cadherin呈显著高表达，而E-cadherin呈显著低表达，三者可能通过相互作用共同参与NSCLC的发生和发展。
목적：탐토상피형개점부소（E-cadherin）、신경형개점부소（N-cadherin）급Snail재비소세포폐암（NSCLC）중적표체급기림상의의。방법채용실시형광정량PCR측정Snail、E-cadherin화N-cadherin기인재10대비소세포폐암급기암방정상폐조직중적표체정황，동시채용면역조화법검측105례NSCLC이급41례암방조직중Snail、E-cadherin화N-cadherin단백적표체。결과여암방정상폐조직상비교，비소세포폐암조직중E-cadherin적표체현저감소，N-cadherin화Snail표체명현증가（P<0.05）。NSCLC조직중，Snail적표체여N-cadherin적표체정명현적정상관（P<0.05，r=0.21），여E-cadherin적표체정현저부상관（P<0.05，r=-0.39），이N-cadherinl적표체여E-cadherin적표체야정명현부상관（P<0.05，r=-0.53）。결론비소세포폐암조직중， Snail、N-cadherin정현저고표체，이E-cadherin정현저저표체，삼자가능통과상호작용공동삼여NSCLC적발생화발전。
Objective To investigate the expressions of snail, E-cadherin and N-cadherin in non-small cell lung can-cer (NSCLC) and their clinical significance. Methods The quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used to detect the Snail, E-cadherin and N-cadherin mRNA levels in 10 cases of NSCLC tis-sues and the tissues adjacent to carcinoma. Then the immunohistochemistry (IHC) was applied to detect the protein levels of Snail, E-cadherin and N-cadherin in 105 cases of non-small cell lung cancer and 41benign pulmonary tissues. Results Compared with the benign pulmonary tissues, the mRNA and protein expression of E-cadherin in NSCLC tissues were significantly decreased, while the mRNA and protein expression of Snail and N-cadherin were both obviously increased (P<0.05). In NSCLC tissues, the Snail expression was positively correlated with N-cadherin expression (P<0.05, r=0.21) and negatively correlated with E-cadherin expression (P<0.05, r=-0.39), and the N-cadherin expression was also nega-tively correlated with E-cadherin expression (P<0.05, r=-0.53). Conclusion In NSCLC tissues, the expressions of Snail and N-cadherin were both up-regulated, while the E-cadherin expressions were significantly down-regulated. All of them may be involved in the occurrence and development of NSCLC through their interaction.