CAJ | 학술논문

目的：在传统结缔组织铺片基础上开展脂肪组织油红染色方法在医学本科生组织学实验教学中的应用。方法学生先进行疏松结缔组织铺片，并施行脂肪组织油红 o-甲苯胺兰-伊红三重染色，然后镜下观察。结果油红 o 染色把结缔组织中的脂肪细胞内脂滴保存下来并染上红色。脂肪组织中央的细胞脂滴均匀红染，充满胞浆，周边的脂肪细胞胞浆中油红染色很少，细胞呈空泡状，显示出脂肪细胞亚群存在。甲苯胺兰染色使得疏松结缔组织中肥大细胞染成紫红色，胞核染色浅，细胞数量多、成群分布。伊红可使得结缔组织内除脂肪细胞、肥大细胞意外的其他细胞的胞浆和胶原纤维染成淡红色。结论传统的组织学平铺片技术基础上引入脂肪油红 o-甲苯胺兰-伊红三重染色，可增强学生动手能力，并能很好地了解输送结缔组织中细胞的不同表型和分布，丰富组织学内容，把教学、科研连接一起，达到提高实验教学质量的目的。
목적：재전통결체조직포편기출상개전지방조직유홍염색방법재의학본과생조직학실험교학중적응용。방법학생선진행소송결체조직포편，병시행지방조직유홍 o-갑분알란-이홍삼중염색，연후경하관찰。결과유홍 o 염색파결체조직중적지방세포내지적보존하래병염상홍색。지방조직중앙적세포지적균균홍염，충만포장，주변적지방세포포장중유홍염색흔소，세포정공포상，현시출지방세포아군존재。갑분알란염색사득소송결체조직중비대세포염성자홍색，포핵염색천，세포수량다、성군분포。이홍가사득결체조직내제지방세포、비대세포의외적기타세포적포장화효원섬유염성담홍색。결론전통적조직학평포편기술기출상인입지방유홍 o-갑분알란-이홍삼중염색，가증강학생동수능력，병능흔호지료해수송결체조직중세포적불동표형화분포，봉부조직학내용，파교학、과연련접일기，체도제고실험교학질량적목적。
Objective To establish a method to detect adipose tissue in the connective tissue for ex-perimental teaching of histology and embryology of medical undergraduates based on the traditional con-nective tissue spread .Methods After loose connective tissue spread adipose tissue was stained with oil red-toluidine blue-eosin triple staining ,and observed by the optic microscopy .Results The fat droplets in the adipose cells of the connective tissue with oil red staining represented red color .The adipose cells located in the center of adipose tissue evenly filled with red lipid droplets ,while the adipose cells located in the pe-riphery of adipose tissue demonstrated no fat droplets ,indicating different phenotypes of fat cells existed in adipose tissue .Toluidine blue staining demonstrated the clustered mast cells in the loose connective tis-sue in blue-colored cytoplasma ,and light nuclei .The other cells and collagen fibers in the connective tis-sue stained by eosin represented pink .Conclusion The oil red-toluidine blue-eosin triply staining can clearly represent more cell types ,e .g .mast cells ,and adipose cells in the loose connective tissue than other stai-ning methods .Students performed the experiment by themselves can easily master the more useful experi-mental skills and can better understand the different phenotype and the distribution of cells in the loose connective tissue .This might reduce the gap between basic medical teaching and scientific research and a-chieve the goal of improving the quality of experimental teaching .