中国马铃薯
中國馬鈴藷
중국마령서
CHINESE POTATO
2014年
4期
233-234
,共2页
往返电泳%马铃薯类病毒%技术要点
往返電泳%馬鈴藷類病毒%技術要點
왕반전영%마령서류병독%기술요점
return-polyacrylamide gel electrophoresis (R-PAGE)%potato spindle tuber viroid (PSTVd)%technical point
目前国内普遍采用的往返电泳法是检测马铃薯类病毒的主要方法之一，但该方法存在灵敏度低，重复性差等问题。因此，针对上述问题总结出一些技术要点：（1）保证植物生长环境及正确取样；（2）提高凝胶中RNA的浓度和质量；（3）控制好反向电泳的温度和时间；（4）及时更换染色液。
目前國內普遍採用的往返電泳法是檢測馬鈴藷類病毒的主要方法之一，但該方法存在靈敏度低，重複性差等問題。因此，針對上述問題總結齣一些技術要點：（1）保證植物生長環境及正確取樣；（2）提高凝膠中RNA的濃度和質量；（3）控製好反嚮電泳的溫度和時間；（4）及時更換染色液。
목전국내보편채용적왕반전영법시검측마령서류병독적주요방법지일，단해방법존재령민도저，중복성차등문제。인차，침대상술문제총결출일사기술요점：（1）보증식물생장배경급정학취양；（2）제고응효중RNA적농도화질량；（3）공제호반향전영적온도화시간；（4）급시경환염색액。
At present, return-polyacrylamide gel electrophoresis (R-PAGE) is a commonly used method for potato spindle tuber viroid (PSTVd) detection in China. But, there are some drawbacks in this technique, such as low sensitivity and poor repeatability. In response to the problems mentioned above, some technical points were pointed out based on the author's experience for PSTVd detection:(1) optimization of plant growth environments and proper sampling;(2) increasing the concentration and quality of RNA in the gel;(3) the control of temperature and time in reverse electrophoresis;and (4) replacing the staining solution timely.
