辽宁医学院学报
遼寧醫學院學報
료녕의학원학보
JOURNAL OF LIAONING MEDICAL UNIVERSITY
2014年
4期
30-32
,共3页
结直肠癌%细胞角蛋白CK20%微转移%T7-RNA聚合酶
結直腸癌%細胞角蛋白CK20%微轉移%T7-RNA聚閤酶
결직장암%세포각단백CK20%미전이%T7-RNA취합매
colorectal cancer%cytokeratin CK20%micro-metastases%T7-RNA polymerase
目的：检测患者术前外周血和腹水中细胞角蛋白(CK20)的水平对结直肠癌微转移的意义。方法采用改进的T7-RNA聚合酶催化的荧光扩增法( FACTT-T7 RNA),通过与酶联免疫法( ELISA)和流式细胞学技术( FCM)的对比,对附属第三医院2012年11月至2013年11月收治的56例结直肠癌患者术前外周血和术中腹水的CK20水平进行检测评价。结果56例结直肠癌患者术中,外周血CK20阳性率FACTT为57.14%(32/56), ELISA为25.00%(14/56)；腹水CK20阳性率FACTT为30.36%(17/56), FCM为14.29%(8/56),组内对比差异具有统计学意义(P<0.05)； FACTT方法检测外周血联合腹水CK20阳性检出率达73.21%(41/56),组间对比率差异具有显著统计学意义(分别为P<0.05, P<0.01), CK20的表达与肿瘤TNM分期、分化程度有关( P<0.05)。结论 FACTT方法联合检测外周血和腹水中CK20水平的阳性表达率高,对结直肠癌患者的微转移具有重要的临床意义。
目的：檢測患者術前外週血和腹水中細胞角蛋白(CK20)的水平對結直腸癌微轉移的意義。方法採用改進的T7-RNA聚閤酶催化的熒光擴增法( FACTT-T7 RNA),通過與酶聯免疫法( ELISA)和流式細胞學技術( FCM)的對比,對附屬第三醫院2012年11月至2013年11月收治的56例結直腸癌患者術前外週血和術中腹水的CK20水平進行檢測評價。結果56例結直腸癌患者術中,外週血CK20暘性率FACTT為57.14%(32/56), ELISA為25.00%(14/56)；腹水CK20暘性率FACTT為30.36%(17/56), FCM為14.29%(8/56),組內對比差異具有統計學意義(P<0.05)； FACTT方法檢測外週血聯閤腹水CK20暘性檢齣率達73.21%(41/56),組間對比率差異具有顯著統計學意義(分彆為P<0.05, P<0.01), CK20的錶達與腫瘤TNM分期、分化程度有關( P<0.05)。結論 FACTT方法聯閤檢測外週血和腹水中CK20水平的暘性錶達率高,對結直腸癌患者的微轉移具有重要的臨床意義。
목적：검측환자술전외주혈화복수중세포각단백(CK20)적수평대결직장암미전이적의의。방법채용개진적T7-RNA취합매최화적형광확증법( FACTT-T7 RNA),통과여매련면역법( ELISA)화류식세포학기술( FCM)적대비,대부속제삼의원2012년11월지2013년11월수치적56례결직장암환자술전외주혈화술중복수적CK20수평진행검측평개。결과56례결직장암환자술중,외주혈CK20양성솔FACTT위57.14%(32/56), ELISA위25.00%(14/56)；복수CK20양성솔FACTT위30.36%(17/56), FCM위14.29%(8/56),조내대비차이구유통계학의의(P<0.05)； FACTT방법검측외주혈연합복수CK20양성검출솔체73.21%(41/56),조간대비솔차이구유현저통계학의의(분별위P<0.05, P<0.01), CK20적표체여종류TNM분기、분화정도유관( P<0.05)。결론 FACTT방법연합검측외주혈화복수중CK20수평적양성표체솔고,대결직장암환자적미전이구유중요적림상의의。
Objective To detect the level of CK20 in peripheral blood and ascites and its implications on micrometastases of pa-tients with colorectal cancer. Methods The improved fluorescent amplification catalyzed byT7 polymerase technique ( FACTT-T7 RNA) was adopted and through the comparison between the enzyme-linked immunosorbent assay ( ELISA) and flow cytometry tech-niques ( FCM) , CK20 levels of 56 patients with colorectal cancer from Nov. 2012 to Nov. 2013 was evaluated by preoperative blood and ascites. Results 56 patients with colorectal cancer, peripheral blood CK20 FACTT positive rate was 57.14% ( 32/56 ) , and ELISA was 25.00% (14/56), ascites CK20 FACTT positive rate was 30.36% (17/56), and FCM was 14.29% (8/56), the differ-ences between the two groups was of statistically significance (P<0.05), by FACTT technique, peripheral and ascites CK20 positive rate was 73.21% (41/56), with significant difference (P<0.05, P<0.01) between the two groups. The level of CK20 expression re-lated to TNM stage and Degree of differentiation (P<0.05). Conclusion Positive expression of CK20 levels in peripheral blood and ascites was high through FACTT combined detection, which is of clinical significance to micrometastasis.
