CAJ | 학술논문

葡萄（Vitis vinifera L 。）籽蛋白是葡萄籽的重要组分之一，具有开发成膳食蛋白或功能性蛋白的潜力。在确定葡萄籽各主要成分和金属元素含量的基础上，通过双向电泳结合质谱鉴定手段，确定了葡萄籽蛋白的主要成分为11 S球蛋白；通过氨基酸组成检测及与大豆分离蛋白的比较，明确了葡萄籽蛋白的营养价值；最后利用红外光谱分析，对葡萄籽蛋白的二级结构进行了初步分析。研究结果将为葡萄籽蛋白的综合利用及功能性产品的研发提供理论基础。
포도（Vitis vinifera L 。）자단백시포도자적중요조분지일，구유개발성선식단백혹공능성단백적잠력。재학정포도자각주요성분화금속원소함량적기출상，통과쌍향전영결합질보감정수단，학정료포도자단백적주요성분위11 S구단백；통과안기산조성검측급여대두분리단백적비교，명학료포도자단백적영양개치；최후이용홍외광보분석，대포도자단백적이급결구진행료초보분석。연구결과장위포도자단백적종합이용급공능성산품적연발제공이론기출。
As one of the important components in grape (Vitis vinifera L .) ,seeds protein has a potential for developing into dietary or functional protein .Basing on the determination of the contents of the main ingredients and metal elements in grape seeds ,11S globulin was confirmed as the main component of grape seeds protein by two-dimensional electrophoresis coupled with mass spectrometry identification .Then ,nutritive values of grape seeds protein were determined by amino acid composition detection and the comparison with those of soybean protein isolate .Finally ,the secondary structure of grape seeds protein was preliminary speculated by infrared spectroscopic analysis .The results will provide theoretical basis for the comprehensive utilization and developing functional product of grape seeds protein .