CAJ | 학술논문

以自制磁性琼脂糖微球为基质，环氧氯丙烷为活化剂，亚氨基乙二酸作为螯合剂，制备了表面螯合Ni2＋的磁性凝胶微球（Mag-Agarose-Ni）．IR结果表明Ni2＋成功螯合到了磁性凝琼脂糖胶微球上；SEM 结果显示在螯合Ni2＋后，M ag-Agarose-Ni形貌没有发生明显变化，且平均粒径为23μm ；原子吸收光谱结果表明M ag-Agarose-Ni表面螯合的Ni2＋的量为2．12×10-5 mol/mg ；磁性能测试表明M ag-Agarose-Ni具有超顺磁性，其磁饱和强度为20．8 emu/g ，具有良好的磁响应性．将Mag-Agarose-Ni用于六聚组氨酸融合蛋白K8的纯化研究， SDS-PAGE结果表明 Mag-Agarose-Ni较市售 Ni-NTA对K8具有更优的亲和分离效果，经15 min的孵育后， Mag-Agarose-Ni对K8的吸附容量可达到8．8μg/mg ．
이자제자성경지당미구위기질，배양록병완위활화제，아안기을이산작위오합제，제비료표면오합Ni2＋적자성응효미구（Mag-Agarose-Ni）．IR결과표명Ni2＋성공오합도료자성응경지당효미구상；SEM 결과현시재오합Ni2＋후，M ag-Agarose-Ni형모몰유발생명현변화，차평균립경위23μm ；원자흡수광보결과표명M ag-Agarose-Ni표면오합적Ni2＋적량위2．12×10-5 mol/mg ；자성능측시표명M ag-Agarose-Ni구유초순자성，기자포화강도위20．8 emu/g ，구유량호적자향응성．장Mag-Agarose-Ni용우륙취조안산융합단백K8적순화연구， SDS-PAGE결과표명 Mag-Agarose-Ni교시수 Ni-NTA대K8구유경우적친화분리효과，경15 min적부육후， Mag-Agarose-Ni대K8적흡부용량가체도8．8μg/mg ．
Magnetic Ni2+ chelate affinity agarose microspheres (Mag-Agarose-Ni) were prepared by introducing nickel ions into the surface of self-made Magnetic Agarose Microspheres with the aid of iminodiacetic acid as chelating agent .The results of Fourier transform infrared (FTIR) indicated that Ni2+ had successfully chelated on the magnetic agarose gel microspheres .The analysis of scanning electron micrographs (SEM ) showed that the size of Mag-Agarose-Ni had no significant change after the chelation with Ni2+ ,and the average particle diameter was 23 μm .The atomic absorption spectroscopy confirmed the amount of chelated Ni2+ on the surface of magnetic agarose microspheres was 2 .12 × 10-5 mol/mg .The magnetic measurement revealed that the resultant Mag-Agarose-Ni were superparamagnetic with a saturation magnetization of 20 .8 emu/g .Mag-Agarose-Ni was used as a magnetic affinity separation support for 6 × His-tagged Proteins K8 .The results of SDS-PAGE showed that Mag-Agarose-Ni had better affinity to K8 than commercial Ni-NTA , and the adsorption capacity of K8 by Mag-Agarose-Ni was 8 .8 μg/mg after 15 minutes incubation .