江西林业科技
江西林業科技
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JIANGXI FORESTRY SCIENCE AND TECHNOLOGY
2014年
4期
1-3,19
,共4页
邹娜%曹福亮%张露%汪贵斌%陈周琴%刘旭忻
鄒娜%曹福亮%張露%汪貴斌%陳週琴%劉旭忻
추나%조복량%장로%왕귀빈%진주금%류욱흔
龙脑樟%愈伤组织%诱导%增殖
龍腦樟%愈傷組織%誘導%增殖
용뇌장%유상조직%유도%증식
Cinnamomum camphora%callus%induction%proliferation
以龙脑樟的茎段作为外植体，进行腋芽的诱导获得无菌苗，继而腋芽小叶被用于愈伤组织的诱导并进行增殖培养。结果表明外植体适宜的灭菌方法为：0.1%升汞灭菌10 min，污染率为21.6%；腋芽小叶愈伤组织诱导最佳培养基配方为：MS+BA 4.0 mg/L+NAA 0.1 mg/L，诱导率可达72.7%，较佳增殖培养基组合为：MS+BA 5.0 mg/L+NAA 0.5 mg/L。
以龍腦樟的莖段作為外植體，進行腋芽的誘導穫得無菌苗，繼而腋芽小葉被用于愈傷組織的誘導併進行增殖培養。結果錶明外植體適宜的滅菌方法為：0.1%升汞滅菌10 min，汙染率為21.6%；腋芽小葉愈傷組織誘導最佳培養基配方為：MS+BA 4.0 mg/L+NAA 0.1 mg/L，誘導率可達72.7%，較佳增殖培養基組閤為：MS+BA 5.0 mg/L+NAA 0.5 mg/L。
이용뇌장적경단작위외식체，진행액아적유도획득무균묘，계이액아소협피용우유상조직적유도병진행증식배양。결과표명외식체괄의적멸균방법위：0.1%승홍멸균10 min，오염솔위21.6%；액아소협유상조직유도최가배양기배방위：MS+BA 4.0 mg/L+NAA 0.1 mg/L，유도솔가체72.7%，교가증식배양기조합위：MS+BA 5.0 mg/L+NAA 0.5 mg/L。
Stems of Cinnamomum camphora were used as explants to get aseptic seedling and induce axillary bud, and then the young leaf of axillary buds were carried for callus induction and proliferation. The results showed that:the best disinfector for explants sterilization was HgCl2 and for 10 min, with contamination rate is 21.6%. The callus induction rate of young leaf on MS+BA 4.0 mg/L+NAA 0.1 mg/L were 72.7%, and the better callus proliferation medium was:MS+BA 5.0 mg/L+NAA 0.5 mg/L.
