CAJ | 학술논문

设计5组等氮等脂等能的饲料，在室内流水系统进行68d的养殖实验，探讨高植物蛋白饲料中添加不同分子量水解鱼蛋白对大菱鲆Scophthalmus maximus L.[(16.05±0.03) g]幼鱼消化能力的影响。分别在高植物蛋白饲料中添加5.4%超滤水解鱼蛋白(UF)、5.5%未经超滤水解鱼蛋白(FPH)、5.5%超滤截留水解鱼蛋白(RF)，其均占饲料蛋白的10%，以及不添加水解蛋白(PP)，以上各组鱼粉含量均为18%，对照组(FM)鱼粉含量为67.5%。研究结果表明， FM组的大菱鲆特定生长率与UF、FPH及PP组无显著差异(P>0.05)；饲料效率、蛋白质效率和蛋白质沉积率在FM组与UF组无显著差异(P>0.05)，但显著高于FPH、RF及PP组(P<0.05)；饲料干物质和蛋白质消化率在UF、FPH及RF组显著高于PP组(P<0.05)，但显著低于FM组(P<0.05)，在UF组显著高于FPH和RF组(P<0.05)；不同处理对饲料氨基酸和牛磺酸的消化率均产生显著影响(P<0.05)，趋势为FM组最高，其次为UF组， PP组最低；半胱氨酸和牛磺酸的消化率在添加水解鱼蛋白的3组(UF、FPH和RF组)和不添加水解鱼蛋白的2组(FM和PP组)呈相反的趋势。上述结果表明，在高植物蛋白饲料中添加低分子量水解鱼蛋白(UF)，大菱鲆幼鱼的生长和饲料利用有升高的趋势，但UF、FPH以及RF都显著提高了大菱鲆对饲料干物质、蛋白质和氨基酸的消化率，且 UF 效果优于 FPH 和 RF。此外，添加不同分子量水解鱼蛋白都降低了牛磺酸的消化率。
설계5조등담등지등능적사료，재실내류수계통진행68d적양식실험，탐토고식물단백사료중첨가불동분자량수해어단백대대릉평Scophthalmus maximus L.[(16.05±0.03) g]유어소화능력적영향。분별재고식물단백사료중첨가5.4%초려수해어단백(UF)、5.5%미경초려수해어단백(FPH)、5.5%초려절류수해어단백(RF)，기균점사료단백적10%，이급불첨가수해단백(PP)，이상각조어분함량균위18%，대조조(FM)어분함량위67.5%。연구결과표명， FM조적대릉평특정생장솔여UF、FPH급PP조무현저차이(P>0.05)；사료효솔、단백질효솔화단백질침적솔재FM조여UF조무현저차이(P>0.05)，단현저고우FPH、RF급PP조(P<0.05)；사료간물질화단백질소화솔재UF、FPH급RF조현저고우PP조(P<0.05)，단현저저우FM조(P<0.05)，재UF조현저고우FPH화RF조(P<0.05)；불동처리대사료안기산화우광산적소화솔균산생현저영향(P<0.05)，추세위FM조최고，기차위UF조， PP조최저；반광안산화우광산적소화솔재첨가수해어단백적3조(UF、FPH화RF조)화불첨가수해어단백적2조(FM화PP조)정상반적추세。상술결과표명，재고식물단백사료중첨가저분자량수해어단백(UF)，대릉평유어적생장화사료이용유승고적추세，단UF、FPH이급RF도현저제고료대릉평대사료간물질、단백질화안기산적소화솔，차 UF 효과우우 FPH 화 RF。차외，첨가불동분자량수해어단백도강저료우광산적소화솔。
In this study, we investigated whether and how the molecular weight of fish protein hydrolysate in the plant-protein diets would affect the digestive ability of juvenile turbot (Scophthalmus maximus L). The 68-day experi-ments were conducted on fish with initial weight of (16.05±0.03) g. Five groups of diets (UF, FPH, RF, PP, and FM) were formulated to be isolipidic, isonitrogenous and isoenergetic. UF (ultrafiltered fish protein hydrolysate), FPH (fish protein hydrolysate) and RF (retentate fish protein hydrolysate) contained 5.4%, 5.5%and 5.5%of protein hydrolysates respectively (10% of total dietary protein). PP contained zero protein hydrolysate. Fish meal consisted of 18% of the diets in UF, FPH, RF, and PP, and it was the sole protein source in the control diet (FM). The special growth rates of fish fed with UF (UF fish), FPH and PP were not significantly different from FM fish (P>0.05). There was also no difference in feed efficiency (FE), protein efficiency ratio (PER), or protein productive value (PPV) between UF fish and FM fish (P>0.05). However FPH fish, RF fish, and PP fish displayed significantly lower FE, PER and PPV than FM fish did (P<0.05). The apparent digestibility coefficients (ADC) for the dry matter and protein of UF, FPH and RF fish were significantly higher than that of PP fish (P<0.05), but lower than that of FM fish (P<0.05). Between UF, FPH, and RF fish, the ADC for the dry matter and protein of UF was significantly higher than that of the latter two (P<0.05). The ADCs for 16 amino acids and taurine could be obviously affected by different dietary treatments (P<0.05). For most amino acids the ADC was the highest in FM fish and the lowest in PP fish among all the groups. The ADCs for cysteine and taurine exhibited opposite patterns between the fish protein hydrolysates-containing groups (UF, FPH and RF) and the fish protein hydrolysates-lacking groups (FM and PP). In conclusion, addition of UF and FPH to the high plant pro-tein diets could help increase the growth and feed utilization of the juvenile turbot;the ACD for dry matter, protein, and amino acids could be improved with FPH and UF; compared to FPH and RF, the low molecular weight UF could be more effective to improve the growth. However, it seemed that the fish protein hydrolysate might reduce the ACD for taurine.