CAJ | 학술논문

以实验室养殖的异育银鲫(Carassius auratus gibelio)“中科3号”成鱼为对象，利用PCR-DGGE技术比较研究了其经饥饿与恢复投喂后消化道微生物多样性的变化。实验共检测到23条谱带，其中饥饿组、投喂组谱带数分别为18条和17条(共有谱带12条)，投喂组大部分谱带亮度高于饥饿组。基于PCR-DGGE所得到的谱带进行的 CA (Correspondence Analysis)分析和聚类都表明饥饿组与投喂组消化道微生物存在明显不同。结果表明，在充分饥饿处理后，恢复饲料投喂可导致消化道微生物种类减少及微生物平均密度的增加，同时对消化道微生态系统内微生物种类组成有显著影响。研究为进一步阐述消化道微生物功能基因奠定了基础，也为水产鱼类肠道菌群研究提供一些基础数据。
이실험실양식적이육은즉(Carassius auratus gibelio)“중과3호”성어위대상，이용PCR-DGGE기술비교연구료기경기아여회복투위후소화도미생물다양성적변화。실험공검측도23조보대，기중기아조、투위조보대수분별위18조화17조(공유보대12조)，투위조대부분보대량도고우기아조。기우PCR-DGGE소득도적보대진행적 CA (Correspondence Analysis)분석화취류도표명기아조여투위조소화도미생물존재명현불동。결과표명，재충분기아처리후，회복사료투위가도치소화도미생물충류감소급미생물평균밀도적증가，동시대소화도미생태계통내미생물충류조성유현저영향。연구위진일보천술소화도미생물공능기인전정료기출，야위수산어류장도균군연구제공일사기출수거。
In this study, we applied PCR-DGGE fingerprinting to investigate the effects of refeeding on the diversity of intestinal microflora in allogynogenetic crucian carp. Fishes were reared in a semi-recirculating system until maturation at Institute of Hydrobiology. After the 30-day starvation, fishes were divided into two groups (the hungry group and the feed group). Three fishes were blindly selected from the hungry group for the extraction of the microbial DNA with the traditional Phenol-chloroform method. In the meanwhile, fishes in the feed group resumed to be fed. Three fishes in the feed group were blindly selected for extraction of the microbial DNA after 25 days. The PCR-generated bacterial DNA products were separated using a polyacrylamide gel as it exhibited increasingly higher concentrations of chemical denaturant. Correspondence Analysis (CA) and clustering based on Jaccard index was used to analyze the similarity of the samples. The results showed that the feed group displayed less bands than the hungry group. There was significant diffe-rence in the deleted bands between the feed group (B1, B2, B5, B6, B7 and B23) and the hungry group (B11, B14, B16, B17 and B21), so we reasoned that the similarity of microflora between the two groups was low. The variance analysis showed that the variance within the feed group was larger than that within the hungry group. In conclusion, our results suggested that refeeding had strong effects on the diversity of intestinal microflora in allogynogenetic crucian carp.