CAJ | 학술논문

采用草鱼呼肠孤病毒腹腔注射草鱼,通过定量 RT-PCR 检测了12个抗病毒免疫相关基因在鳃中不同时间点的表达模式,以了解鳃对内源性病毒的免疫应答。模式识别受体基因 CiTLR3、CiTLR7、CiTLR22、CiRIG-I、CiMDA5、CiLGP2、CiNOD1和CiNOD2,以及干扰素基因CiIFN-I的表达在注射病毒后12h、24h、48h及72h基本都上调。IgM基因的表达仅在72h上调。接头分子CiMyD88和CiIPS-1基因的表达在早期下调(6h),然后逐渐上升。为了证实病毒感染的可靠性,通过RT-PCR检测了病毒VP4基因。结果表明草鱼鳃在抗病毒免疫方面发挥着重要作用。
채용초어호장고병독복강주사초어,통과정량 RT-PCR 검측료12개항병독면역상관기인재새중불동시간점적표체모식,이료해새대내원성병독적면역응답。모식식별수체기인 CiTLR3、CiTLR7、CiTLR22、CiRIG-I、CiMDA5、CiLGP2、CiNOD1화CiNOD2,이급간우소기인CiIFN-I적표체재주사병독후12h、24h、48h급72h기본도상조。IgM기인적표체부재72h상조。접두분자CiMyD88화CiIPS-1기인적표체재조기하조(6h),연후축점상승。위료증실병독감염적가고성,통과RT-PCR검측료병독VP4기인。결과표명초어새재항병독면역방면발휘착중요작용。
Gill plays an important physical barrier role in defending environmental microbes. How are im-mune responses to endogenous viruses in gill? In the present study, mRNA expressions of 12 antiviral im-mune-related genes were examined by quantitative real-time RT-PCR (qRT-PCR) in grass carp (Cteno-pharyngodon idella) gill after grass carp reovirus (GCRV) challenge. The relative values of CiTLR3, CiTLR7, CiTLR22, CiRIG-I, CiMDA5, CiLGP2, CiNOD1, CiNOD2 and CiIFN-I were almost up-regulated at 12h, 24h, 48h and 72h. Additionally, the mRNA expression of CiIgM was triggered at 72h. However, relative expres-sions of CiMyD88 and CiIPS-1 were down-regulated at 6h, and subsequently increased. To further verify the reliability of viral infection, VP4 gene (outer capsid protein of GCRV, segment 6) was checked by RT-PCR amplification. The results indicate that gill serves as an important immune organ, and plays crucial roles in triggering antiviral immune responses in grass carp.