畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2014年
7期
14-15
,共2页
黄洋%靳辉%薛艳蓉%靳光
黃洋%靳輝%薛豔蓉%靳光
황양%근휘%설염용%근광
绵羊%耳成纤维细胞%G418
綿羊%耳成纖維細胞%G418
면양%이성섬유세포%G418
sheep%ear fibroblast cells%G418
旨在探寻10~14 d内G418致死绵羊耳成纤维细胞的最低浓度。试验设定了100~1000μg/mL的10个G418浓度梯度,加入绵羊耳成纤维细胞培养体系内,培养14 d。结果表明,当G418浓度为200~400μg/mL时,在10~14 d内能致死全部绵羊耳成纤维细胞。
旨在探尋10~14 d內G418緻死綿羊耳成纖維細胞的最低濃度。試驗設定瞭100~1000μg/mL的10箇G418濃度梯度,加入綿羊耳成纖維細胞培養體繫內,培養14 d。結果錶明,噹G418濃度為200~400μg/mL時,在10~14 d內能緻死全部綿羊耳成纖維細胞。
지재탐심10~14 d내G418치사면양이성섬유세포적최저농도。시험설정료100~1000μg/mL적10개G418농도제도,가입면양이성섬유세포배양체계내,배양14 d。결과표명,당G418농도위200~400μg/mL시,재10~14 d내능치사전부면양이성섬유세포。
In order to find the lowest concentration of G418 to kill ear fibroblast cells of sheep from 10 days to 14 days, effects of G418 of 10 concentrations at 100-1 000 μg/mL on ear fibroblast cells of sheep were studied. Results showed that the G418 at 200-400μg/mL could kill all ear fibroblast cells of sheep from 10 days to 14 days.