北京市大鼠、小鼠、豚鼠的病理学调查
북경시대서、소서、돈서적병이학조사
Pathologic investigation of rats、mice and cavies in Beijing
  • 万方数据
    中国比较医学杂志 중국비교의학잡지
    CHINESE JOURNAL OF COMPARATIVE MEDICINE
    2014年 7期 54-56 ,共3页

    付永瑶%吕悦%王继宏%姚皓%赵文杰%宋志琦%薛志新%袁振%刘春法%彭云%李超%赵德明%杨利峰

    부영요%려열%왕계굉%요호%조문걸%송지기%설지신%원진%류춘법%팽운%리초%조덕명%양리봉

    啮齿类实验用鼠%病理学诊断%脂肪变性%油红“O”染色%PAS染色 齧齒類實驗用鼠%病理學診斷%脂肪變性%油紅“O”染色%PAS染色
    교치류실험용서%병이학진단%지방변성%유홍“O”염색%PAS염색
    Experimental mice%Pathological diagnosis%Hepatic steatosis%Oil red“O” staining%PAS staining
    目的：实验动物的健康状况对科学研究的发展有重要的影响，为了保证实验用鼠的健康质量，自从2009年起本实验室对北京市多家实验动物单位的实验用鼠进行了病理学抽检，从病理学的角度来调查北京市部分实验鼠的主要脏器的情况。方法我们主要对实验鼠的心、肝、脾、肺、肾、大肠和小肠进行取材，通过甲醛固定，石蜡切片HE染色、冰冻切片油红“O”染色、PAS染色等方法对实验用鼠进行病理学分析。结果根据病理学的调查结果，部分实验动物在以肝脏和肾脏为代表的器官中出现了不同程度的病变。结论结合对实验鼠的微生物学检测，分析认为，北京市的啮齿类实验动物中几乎无细菌、病毒和寄生虫等病原微生物的阳性感染个体，对人、动物及周围环境无明显危害；但是少部分实验鼠在临床上出现一定程度的病理学异常。
    목적：실험동물적건강상황대과학연구적발전유중요적영향，위료보증실험용서적건강질량，자종2009년기본실험실대북경시다가실험동물단위적실험용서진행료병이학추검，종병이학적각도래조사북경시부분실험서적주요장기적정황。방법아문주요대실험서적심、간、비、폐、신、대장화소장진행취재，통과갑철고정，석사절편HE염색、빙동절편유홍“O”염색、PAS염색등방법대실험용서진행병이학분석。결과근거병이학적조사결과，부분실험동물재이간장화신장위대표적기관중출현료불동정도적병변。결론결합대실험서적미생물학검측，분석인위，북경시적교치류실험동물중궤호무세균、병독화기생충등병원미생물적양성감염개체，대인、동물급주위배경무명현위해；단시소부분실험서재림상상출현일정정도적병이학이상。
    Objective To insure the health of experiment mice , since 2009 our lab tool laboratory rodents from Beijing area to investigate the health status of laboratory rodents .We mainly use the pathologic method to investigate experiment mice.Methods The specimens we took from the experimental animals were mainly hearts , livers, spleens, lungs, kidneys, small intestines and large intestines .Specimens were immediately fixed by formalin .HE staining sections, PAS staining sections , oil red “O” staining frozen sections were observed under light microscope .Results According to the pathological examination there are different degree of pathological changes among the organs of laboratory rodents , such as hepatic and kidney .Conclusion Combined with the result of microbial detection , the laboratory rodents are not infectious with Pathogenic microorganism , such as bacteria , virus and parasite .And they have no harm to human beings , other animals and the surrounding environment .But small parts of laboratory rodents show certain extents pathological anomaly in clinically .
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